Amplification and detection of shigella spp. and enteroinvasive strains of Escherichia coli
AssigneeBecton, Dickinson and Company
InventorsTobin J. Hellyer, Ray A. McMillian
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Abstract
Amplification primers and methods for specific amplification and detection of a Shigella spp. and enteroinvasive stains of Escherichia coli (EIEC) target are disclosed. The primer-target binding sequences are useful for amplification and detection of Shigella and EIEC target in a variety of amplification and detection reactions.
Claims11
An oligonucleotide consisting of a target binding sequence selected from the group consisting of the target binding sequences of ShH1AL48 (SEQ ID NO:1), ShH1AL46 (SEQ ID NO:2), SbH1AL44 (SEQ ID NO:3), of ShH1AR50 (SEQ ID NO:4), ShH1AR46 (SEQ ID NO:5) and ShH1AR42 (SEQ ID NO:6), and optionally, a sequence required for an amplification reaction.
An oligonucleotide consisting of ShH1BL44 (SEQ ID NO:7) or ShH1BBR44 (SEQ ID NO:8).
An oligonucleotide selected from the group consisting of Sh1DL44 (SEQ ID NO:9), a nucleic acid complementary to SEQ ID NO:9, Sh1DR46 (SEQ ID NO:10) and a nucleic acid complementary to SEQ ID NO:
- 10.
A pair of amplification primers comprising:
- a) a first primer consisting of a target binding sequence selected from the group consisting of the target binding sequences of ShH1AL48 (SEQ ID NO:1), ShH1AL46 (SEQ ID NO:2), ShH1AL44 (SEQ ID NO:3), and, optionally, a sequence required for an amplification reaction, and;
- b) a second primer consisting of a target binding sequence selected from the group consisting of the target binding sequences of ShH1AR50 (SEQ ID NO:4), ShH1AR46 (SEQ ID NO:5) and ShH1AR42 (SEQ ID NO:6), and, optionally, a sequence required for an amplification reaction.
Dependencies are inferred from the claim text.