Fish insulin-like growth factor II promoter
AssigneeAcademia Sinica
InventorsJen-Leih Wu, Jyh-Yih Chen
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Abstract
This present invention relates to the findings of the DNA sequences of fish insulin-like growth factor II (IGF-II) promoter regions and recombinant IGF-II promoters. These DNA sequences are capable of being expressed in eukaryotic cells and fish embryos of another fish species. The integration of the IGF-II promoter regions or recombinant IGF-II promoters into fish of another species results in the creation of a transgenic fish. The results of this invention illustrate that a fish IGF-II promoter not only can act as a growth factor to stimulate the growth and development of fish, but also is capable of being expressed in other eukaryotic cells such as in human cells.
Claims18
A recombinant DNA molecule comprising:
- a fish insulin-like growth factor II (IGF-II) promoter region having the DNA sequence of SEQ ID NO:1;
- and
- a coding sequence encoding a green fluorescence protein (GFP).
A recombinant DNA molecule comprising:
- an IGF-II promoter fragment, wherein said IGF-II promoter fragment has the DNA sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6;
- and
- a coding sequence encoding a green fluorescence protein (GFP).
A recombinant DNA molecule comprising:
- an IGF-II promoter region having the DNA sequence of SEQ ID NO:13;
- and
- a coding sequence encoding an green fluorescence protein (GFP).
Dependencies are inferred from the claim text.