Method for the separation and purification of immunoglobulins
AssigneeTecnogen S.C.p.A.
InventorsGiorgio Fassina, Antonio Verdoliva, Menotti Ruvo
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Abstract
A peptide of formula (I) (H2N—X1—Thr—X2—CO)n—R (I) where X1 and X2, different one another, are an amino acid residue of arginine or tyrosine in configuration L or D, wherein the hydroxy group of threonine and tyrosine and the guanidine moiety of arginine may be protected by a compound conventionally used in peptide chemistry for protecting the hydroxy group and the guanidine moiety, respectively, n is 1,2, 3 or 4, and R, when n is 2,3 or 4, is a group suitable for forming a dimer, trimer or tetramer, while, when n is 1, R is OH, a single amino acid residue, or a peptide chain containing up to 7 amino acid residues.
Claims16
A method for the separation and purification of immunoglobulins which comprises:
- (i) immobilizing on an affinity chromatography support a compound which non covalently binds to at least one immunoglobulin,
- (ii) packing said affinity chromatography support in a chromatographic column,
- (iii) equilibrating said column with a buffer capable of promoting an interaction between immunoglobulin and the immobilized compound,
- (iv) loading said column with a fluid comprising at least one immunoglobulin,
- (v) washing said column with at least one liquid capable of eluting the impurities without interfering with the interaction between immunoglobulin and the immobilized compound,
- (vi) eluting said immunoglobulin previously adsorbed on the column with a dissociating eluent,
- wherein
- said compound which non covalently binds to at least one immunoglobulin is a compound of formula (I)
- (H2N—X1—Thr—X2—CO)n—R (1)
- where
- X1 and X2, different from one another, are an amino acid residue of arginine or tyrosine in configuration L or D, wherein the hydroxy group of threonine and tyrosine and the guanidine moiety of arginine is optionally protected by a compound conventionally used in peptide chemistry for protecting the hydroxy group and the guanidine moiety, respectively, n is 1,2, 3 or 4, and
- R, when n is 2,3 or 4, is a group suitable for forming a dimer, trimer or tetramer, and when n is 1,
- R is a single amino acid residue, or a peptide chain comprising 2 to 7 amino acid residues.
Dependencies are inferred from the claim text.