US 6,207,459 B1Granted

Method for the analysis of medical samples containing haemoglobin

AssigneeRoche Diagnostics GmbH

InventorsRalph Weisheit, Lieselotte Schellong

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Abstract

The invention concerns a method for the determination of an analyte in a sample containing free haemoglobin in which the determination is carried out by an optical measurement and the value measured for the analyte concentration is mathematically corrected. This method is in particular suitable for determining the parameters total protein, iron and albumin in a medical sample e.g. in a serum or plasma sample. The correction of the measured value for the analyte concentration is achieved by the steps (a) measuring the blank value of the sample to be analysed, (b) measuring the blank value of a haemoglobin-free reference sample, (c) measuring the uncorrected value for the analyte concentration and (d) correcting the value obtained in step (c) by correlation with the values obtained in step (a) and (b) in order to obtain the corrected value for the analyte concentration.

Claims17

Dependent claims

A method for the determination of the concentration of an analyte in a sample containing free haemoglobin by optical measurement wherein the measured value for the analyte concentration is corrected by the steps:

a. optically measuring a blank value of the sample to be analyzed,
b. optically measuring a blank value of a haemoglobin-free reference sample,
c. adding a reagent to the sample to produce a detectable change in the sample indicative of analyte concentration,
d. optically measuring an uncorrected value for the analyte concentration and
e. correcting the uncorrected value obtained in step (d) by correlation with the blank values obtained in steps (a) and (b) in order to obtain a corrected value for the analyte concentration,
wherein the corrected value for the analyte concentration is calculated according to the following relation:
C′sample=Csample−F.E1sample+F.E1reference
in which C′sample is the corrected value for the analyte concentration,
Csample is the uncorrected measured value for the analyte concentration in the sample,
F is a test-specific correction factor,
E1sample is the measured blank value in the sample and E1reference is the measured value in the reference sample.

8 more elements

Dependencies are inferred from the claim text.

Facts

Application: 09/147,288Filed 20 Nov 1998
Priority: DE 196 22 08931 May 1996
Granted: 27 Mar 2001
Term ends: Not estimatedThe source records don’t support an estimate.

Classification5 codes

G01N 33/52
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing: use of…
G01N 33/6827
General methods of protein analysis not limited to specific proteins or families of proteins: total protein determination, e.g. albumin in urine
G01N 33/72
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing:…
G01N 33/84
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing:…