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US 6,207,456 B1Granted
Nucleic acid delivery vehicle
AssigneeOpperbas Holding B.V.
InventorsMoshe Baru, Israel Nur
Life of this patent
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Abstract
A composition includes a liposome which has a polynucleic acid and a peptide capable of disrupting membranes under acidic conditions encapsulated within it. The composition is used for efficient transfer of nucleic acids into cells both in vitro as well as in vivo.
Claims6
A composition comprising:
- i) a polynucleic acid molecule;
- ii) a liposome;
- and
- iii) a peptide comprised of less than about 50 amino acids which disrupts membranes under acidic conditions;
- said peptide and the polynucleic acid molecule being encapsulated within the liposome, wherein said peptide is derived from an influenza virus protein and said peptide comprises SEQ ID NO:4.
2 more elements
A composition comprising:
- i) a polynucleic acid molecule;
- ii) a liposome;
- and
- iii) a peptide comprised of less than about 50 amino acids which disrupts membranes under acidic conditions;
- said peptide and the polynucleic acid molecule being encapsulated within the liposome, wherein said peptide is derived from an influenza virus protein and said peptide comprises SEQ ID NO:1.
2 more elements
A method of introducing nucleic acid molecules to cells comprising:
- (a) encapsulating a nucleic acid molecule with a liposome together with a peptide capable of disrupting membranes under acidic conditions, said peptide being comprised of less than about 50 amino acids, wherein said peptide comprises SEQ ID NO:4;
- and
- (b) delivering said liposomes to the cells.
A method of introducing nucleic acid molecules to cells comprising:
- (a) encapsulating a nucleic acid molecule with a liposome together with a peptide capable of disrupting membranes under acidic conditions, said peptide being comprised of less than about 50 amino acids, wherein said peptide comprises SEQ ID NO:1;
- and
- delivering said liposomes to the cells.
Dependencies are inferred from the claim text.