Method for producing docosahexaenoic acid (DHA) using Pseudomonas sp. ys-180
AssigneeSeong Gu Ryu
InventorsYong Seo Shin, Il Hwan Ryu
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AssigneeSeong Gu Ryu
InventorsYong Seo Shin, Il Hwan Ryu
Estimating the term…
The present invention relates to Pseudomonas sp. YS-180 having a capability of producing a docosahexaenoic acid and a method for the production of DHA using such a microorganism. Pseudomonas sp. YS-180 was isolated and identified from the intestine of fishes by investigating the morphological, culturing, and physiological characteristics of a strain excellent in a DHA-producing capability. By TLC and GC, Pseudomonas sp. YS-180 according to the present invention was confirmed that it produced the greatest amount of DHA when it was shaking-cultured in a culture containing glucose 2% (w/v), a yeast extract 2% (w/v), and sea water 20%(v/v). Optimal conditions for producing DHA were a pH of 6.0, and a temperature of 20° C. Under these conditions a DHA production was 96.7 mg/L, and a biomass growth was 7.8 g/L.
A Pseudomonas sp. YS-180 strain isolated from the intestine of fishes and producing docosahexaenoic acid (DHA).
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