Crystalline protease and method for producing same
AssigneeGenencor International, Inc.
InventorsErnst Hakan Gros, Jerry L. Cunefare
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Abstract
A method for preparing a crystalline protease is provided which comprises preparing an aqueous solution containing the protease enzyme and adding to the aqueous solution sodium sulfate, allowing the crystallization to take place at a temperature between 10° C. and 60° C.
Claims21
A method for the crystallization of a bacterial or fungal protease enzyme comprising:
- (a) preparing an aqueous solution containing said protease enzyme;
- (b) adding sodium sulfate to said aqueous solution in a concentration of between about 0.5% and 10% w/v, wherein step (b) is carried out at a temperature of between about 10° C. and 60° C.;
- and (c) recovering the crystallized bacterial or fungal protease enzyme.
A method for the crystallization of a bacterial or fungal protease enzyme comprising;
- (a) preparing an aqueous solution containing said protease enzyme;
- (b) adding sodium sulfate to said aqueous solution in a concentration of between about 0.5% and 10% w/v, wherein said step (b) is carried out at a temperature of between about 20° C. and 40° C. and at a pH of between about 4.0 and 10.0;
- (c) allowing crystals of said protease enzyme to form;
- and (d) recovering the crystallized bacterial or fungal enzyme.
A method for the crystallization of a bacterial or fungal protease enzyme comprising, (a) preparing a protease enzyme solution by removing cells and suspended solids from a fermentation mixture produced by fermentation of a protease producing bacterium or fungus, (b) forming a concentrated solution by concentrating the protease solution wherein the protease is present in a concentration of 20 g/l to 80 g/l, (c) adding sodium sulfate to said concentrated solution in a concentration of between about 0.5% and 10% w/v, wherein step (c) is carried out at a temperature of between about 10° C. and 60° C. and at a pH of between about 4.0 and 10.0, (d) allowing crystal formation of said protease enzyme;
- and (e) recovering the crystallized protease enzyme.
Dependencies are inferred from the claim text.