Polypeptides having aminolevulinic acid activity and nucleic acids encoding same
AssigneeNovo Nordisk Biotech, Inc.
InventorGreg Gambetta
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Abstract
The present invention relates to isolated polypeptides having 5-aminolevulinic acid synthase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Claims17
An isolated nucleic acid sequence encoding a polypeptide having 5-aminolevulinic acid synthase activity, selected from the group consisting of:
- (a) a nucleic acid sequence encoding a polypeptide having an amino acid sequence which has at least 90% identity with the amino acid sequence of SEQ ID NO. 2;
- (b) a nucleic acid sequence having at least 90% homology with the nucleic acid sequence of SEQ ID NO. 1;
- (c) a nucleic acid sequence which hybridizes under high stringency conditions with (i) the nucleic acid sequence of SEQ ID NO. 1 or (ii) its complementary strand;
- or a subsequence thereof of at least 100 nucleotides;
- (d) a subsequence of (a), (b), or (c), wherein the subsequence encodes a polypeptide fragment which has 5-aminolevulinic acid synthase activity.
An isolated nucleic acid sequence comprising a nucleic acid sequence having at least one mutation in the mature polypeptide coding sequence of SEQ ID NO. 1, in which the mutant nucleic acid sequence encodes a polypeptide having the amino acid sequence of SEQ ID NO. 2.
Dependencies are inferred from the claim text.