Tyrosylprotein sulfotransferases and methods of use thereof
AssigneeThe Board of Regents of the University of Oklahoma
InventorKevin L. Moore
Life of this patent
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Abstract
Tyrosylprotein sulfotransferases and nucleic acids encoding the tyrosylprotein sulfotransferases are described. Dual isotopes of the enzyme and of the nucleic acids encoding said enzymes have been identified in human, mouse and C. elegans. The polypeptides and polynucleotides exhibit a wide range of homologies. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational tyrosine sulfation of proteins or peptides produced within the expression system. The enzymes can be used to sulfate peptides or proteins requiring sulfation.
Claims4
A substantially pure tyrosylprotein sulfotransferase produced by the process comprising:
- providing a host cell transformed or transfected with a vector comprising a polynucleotide encoding a tyrosylprotein sulfotransferase, wherein the polynucleotide is selected from the group consisting of:
- (a) a polynucleotide which hybridizes to a nucleic acid sequence comprising nucleotides 82-1194 of SEQ ID. NO:2, nucleotides 211-1323 of SEQ ID NO:4, nucleotides 198-1331 of SEQ ID NO:6, nucleotides 157-1287 of SEQ ID NO:8, nucleotides 66-1208 of SEQ ID NO:10;
- or nucleotides 1-1077 of SEQ ID NO:12;
- and
- (b) a polynucleotide which hybridizes to a nucleic acid sequence comprising nucleotides 289-1086 of SEQ ID NO:2, nucleotides 418-1215 of SEQ ID NO:4, nucleotides 402-1199 of SEQ ID NO:6, nucleotides 358-1155 of SEQ ID NO:8, nucleotides 273-1064 of SEQ ID NO:10, or nucleotides 256-1029 of SEQ ID NO:12;
- and
- wherein the polynucleotides of (a)and (b) hybridize under hybridization condition comprising incubation at 68° C. overnight followed by washing twice with two times SSC, 0.1% SDS at 22° C., and washing twice with 0.1 times SSC, 0.1% SDS at 50° C.;
- culturing the host cell in a manner which causes the expression of the tyrosylprotein sulfotransferase;
- and
- purifying the tyrosylprotein sulfotransferase from the cultured host cell.
A substantially pure tyrosylprotein sulfotransferase produced by the process comprising:
- providing a host cell transformed or transfected with a vector comprising a polynucleotide encoding a tyrosylprotein sulfotransferase comprising:
- amino acid residues 70-105 and 172-205 of SEQ ID NO:1;
- culturing the host cell in a manner which causes the expression of the tyrosylprotein sulfotransferase;
- and
- purifying the tyrosylprotein sulfotransferase from the cultured host cell.
Dependencies are inferred from the claim text.