Nucleic acids encoding polypeptides having laccase activity
AssigneeNovo Nordisk Biotech, Inc.
InventorsDebbie Sue Yaver, Kimberley M. Brown, Sakari Kauppinen, Torben Halkier
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Abstract
The present invention relates to polypeptides having laccase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides.
Claims5
An isolated nucleic acid sequence encoding a polypeptide having laccase activity obtained by (a) hybridizing a DNA from a Coprinus strain under medium stringency conditions with (i) the mature coding region of SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:32 or (ii) its complementary strand, wherein the medium stringency conditions are defined by prehybridization and hybridization at 42° C. in 5X SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 35% formamide, and wash conditions are defined at 60° C. for 30 minutes in 0.2X SSC, 0.1% SDS, and (b) isolating the nucleic and sequence from the DNA.
Dependencies are inferred from the claim text.