Process for the isolation of clavulanic acid and of pharmaceutically acceptable salts thereof from the fermentation broth of streptomyces sp. P 6621 FERM P 2804
AssigneeLek Pharmaceutical & Chemical Co. d.d.
InventorEgidij Capuder
Life of this patent
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Abstract
A process for the isolation of clavulanic acid and pharmaceutically acceptable salts thereof, such as potassium clavulanate, from the aqueous fermentation broth of a clavulanic acid-producing microorganism comprises the microfiltration of the broth without prior treatment.
Claims24
In a process for the isolation of clavulanic acid or a pharmaceutically acceptable salt thereof from an aqueous fermentation broth of a clavulanic acid-producing microorganism, the improvement comprising, as a first step, continuous microfiltration of the broth to thereby produce a microfiltration filtrate containing the clavulanic acid.
A process for the preparation of a concentrated organic extract of clavulanic acid from an aqueous fermentation broth of a clavulanic acid-producing microorganism, the process comprising the steps of:
- (a) continuous microfiltration of the broth, thereby producing a microfiltration filtrate containing the clavulanic acid;
- (b) optionally ultrafiltering the microfiltration filtrate to produce an ultrafiltration filtrate containing the clavulanic acid;
- (c) concentrating the microfiltration filtrate from step (a) or the ultrafiltration filtrate from step (b), thereby producing a retentate containing the clavulanic acid;
- (d) extracting the retentate with a water-immiscible organic solvent, thereby producing an organic phase containing the clavulanic acid;
- (e) drying the organic phase containing the clavulanic acid;
- and
- (f) concentrating the organic phase containing the clavulanic acid, thereby preparing a concentrated organic extract of clavulanic acid.
Dependencies are inferred from the claim text.