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AssigneeThe MetroHealth System
InventorsArthur M. Brown, Barbara A. Wible, Qing Yang
Estimating the term…
The present invention provides polynucleotides that encode a protein, designated herein as K+ Channel Associated Protein or “KChAP”. It has been determined that expressing polynucleotides that encode KChAP in host cells, along with polynucleotides that encode the Kvα channel subunit Kv 2.1, the Kvα channel subunit Kv 2.2, the Kvα channel subunit Kv 1.3, or the Kvα channel subunit Kv 4.3, increases the number of Kv2.1, Kv 2.2, Kv1.3 or Kv4.3 channels, respectively, in the plasma membrane of such cells. The present invention also relates to a method of making cells that have increased numbers of Kv channels on the plasma membranes thereof and to a method of using such cells as model systems for studying the effect of pharmacological agents on Kv channels, particularly on Kv2.1, Kv 2.2, Kv 1.3, and Kv 4.3 channels. The present invention also relates to the protein KChAP.
An isolated polynucleotide comprising a nucleotide sequence encoding a KChAP protein which binds to Kvα subunits and increases number of Kv channels in transfected cell or a nucleotide sequence complementary to a nucleotide sequence encoding said KChAP protein, wherein said KChAP protein comprises an amino acid sequence which is at least 95% identical to SEQ ID NO:
An isolated polynucleotide comprising a nucleotide sequence encoding a protein selected from the group consisting of a rat KChAP protein which binds to Kvα subunits and increases number of Kv channels in transfected cell comprising an amino acid sequence which is at least 95% identical to SEQ ID NO:2, a human KChAP protein which binds to Kvα subunits and increases number of Kv channels in transfected cell comprising an amino acid sequence which at least 95% identical to SEQ ID NO:
A host cell comprising a polynucleotide encoding a KChAP protein, which binds to Kvα subunits and increases number of Kv channels in transfected cell said KChAP protein comprising an amino acid sequence which is at least 95% identical to SEO ID NO:
A host cell comprising an expression vector comprising a nucleic acid sequence which encodes a rat KChAP protein which binds to Kvα subunits and increases number of Kv channels in transfected cell, wherein said protein comprises an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO:2 or the amino acid sequence of SEQ ID NO:4.
Dependencies are inferred from the claim text.