Bacteriocins
AssigneeTeagasc
InventorsReynolds Paul Ross, Mary Clare Rea, Marie Philippa Ryan, Colin Hill
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Abstract
The present invention relates to a novel anti-microbial agent, more particularly, a novel bacteriocin with nisin-like properties. The bacteriocin is designated lacticin 3147 and has the following properties: a molecular weight of approximately 2.8 kDa; inhibiting activity against lactococci, lactobacilli, enterococci, bacilli, leuconostocs, pediococci, clostridia, staphylococci and streptococci; sensitivity to the proteases trypsin, alpha-chymotrypsin, proteinase K and pronase E but not pepsin; heat-stability; activity at acid pH; and the capability of inhibiting nisin-producing bacterial strains.
Claims36
An isolated naturally occurring bacteriocin designated lacticin 3147 from Lactococcus lactis DPC3147 characterised by
- a molecular weight of approximately 2.8 kDa,
- inhibiting activity against lactococci, lactobacilli, enterococci, bacilli, leuconostocs, pediococci, clostridia, staphylococci and streptococci
- sensitivity to the proteases trypsin, alpha-chymotrypsin, proteinase K and pronase E but not pepsin,
- heat-stability,
- activity at acid pH,
- and the capability of inhibiting nisin-producing bacterial strains.
L. lactis DPC3147 strain as deposited at the National Collection of Industrial and Marine Bacteria, Aberdeen, Scotland, on Apr. 11, 1995 under the Accession No. NCIMB 40716.
An isolated plasmid pMRC01 which comprises gene(s) which encode the bacteriocin designated lacticin 3147, lacticin 3147 immunity gene(s) and phage resistance genes as deposited at the National Collection of Industrial and Marine Bacteria, Aberdeen, Scotland, on Apr. 11, 1995 under Accession No. NCIMB 40716.
Isolated gene(s) which encode lacticin 3147 as deposited at the National Collection of Industrial and Marine Bacteria, Aberdeen, Scotland, on Apr. 11, 1995 under Accession No. NCIMB 40716.
An isolated gene encoding a protein or polypeptide conferring immunity to lacticin 3147 as deposited in the plasmid pMRCO1 as deposited at the National Collection of Industrial and Marine Bacteria, Aberdeen, Scotland, on Apr. 11, 1995 under Accession No. NCIMB 40716.
An isolated gene encoding a protein or polypeptide which confers resistance to a phage and as deposited at the National Collection of Industrial and Marine Bacteria, Aberdeen, Scotland, on Apr. 11, 1995 under the Accession No. NCIMB 40716.
The isolated bacteriocin-encoding gene of L. lactis strain DPC2949 as deposited at the National Collection of Industrial and marine Bacteria, Aberdeen, Scotland on Apr. 11, 1995 under the Accession No. NCIMB 40715.
Dependencies are inferred from the claim text.