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US 6,207,402 B1Granted
Detection of mammalian heparanase activity and purification of mammalian heparanase
AssigneeThe Australian National University Act
InventorsCraig Geoffrey Freeman, Christopher Richard Parish
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Abstract
A method for detecting mammalian heparanase activity in a sample such as mammalian tissue, cells or bodily fluids; and a method for the purification of mammalian heparanase from a heparanase-containing material, such as human platelets.
Claims22
A method for the detection of mammalian heparanase activity in a sample, which comprises the steps of:
- i. contacting the sample to be tested with a heparanase substrate for a time and under conditions sufficient for heparanase in the sample to degrade the heparanase substrate;
- ii. separating degradation products from undegraded or partially degraded heparanase substrate by binding the undegraded or partially degraded heparanase substrate with a heparan sulphate-binding protein which is a histidine-rich glycoprotein;
- and
- iii. detecting separated degradation products to indicate heparanase activity in the sample.
1 more element
A method for the detection of mammalian heparanase activity in a sample, which comprises the steps of:
- iv. contacting the sample to be tested with a heparanase substrate for a time and under conditions sufficient for heparanase in the sample to degrade the heparanase substrate;
- v. separating degradation products from undegraded or partially degraded heparanase substrate by binding the undegraded or partially degraded heparanase substrate with a heparan sulphate-binding protein;
- and
- vi. detecting separated degradation products to indicate heparanase activity in the sample;
- wherein said heparan sulphate-binding protein is chicken histidine-rich glycoprotein.
2 more elements
Dependencies are inferred from the claim text.