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US 6,207,398 B1Granted
Cyclosporine derivatives and uses thereof
AssigneeDade Behring Inc.
InventorChengrong Wang
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Abstract
The present invention provides novel cyclosporine C (CsC) derivatives having improved protein conjugatibility and hydrolytic stability. The present invention further provides a CsC derivative conjugated to a carrier, e.g., a solid support. Preferably, the solid support is a latex or magnetic particle.
Claims11
An enzyme-linked immunoassay for the measurement of cyclosporin A levels in whole blood samples comprising the steps of:
- (a) lysing red blood cells in a sample of whole blood containing cyclosporin A;
- (b) contacting the lysed whole blood sample with excess labeled anti-cyclosporin antibody, to form a labeled antibody-cyclosporin A complex;
- (c) separating unbound antibody from the complex by contacting the mixture formed in step (b) with a solid phase comprising a cyclosporin derivative having the structure:
- wherein X is selected from the group consisting of:
- wherein Y is selected from the group consisting of:
- wherein Z is selected from the group consisting of:
- wherein R1 and R2 are each a C1-C8 alkyl group;
- wherein R3 is a bond or C1-C8 alkyl group;
- and
- wherein m is 1-200,
- immobilized on a solid support;
- and
- (d) determining the amount of the label in the complex as a measure of cyclosporin A.
10 more elements
A composition of matter which comprises a conjugate of a carrier and a compound selected from the group consisting of:
- wherein X is selected from the group consisting of:
- wherein Y is selected from from the group consisting of:
- wherein Z is selected from the group consisting of:
- wherein R1 and R2 are each a C1-C8 alkyl group;
- wherein R3 is a bond or C1-C8 alkyl group;
- and
- wherein m is 1-200,
- wherein n is 2-3;
- m is 2-5 and p is 2-3, and
- wherein n is 2-3;
- m is 2-5;
- p is 2-3 and k is 2-3.
9 more elements
Dependencies are inferred from the claim text.