Diagnostic assays for detection of Entamoeba histolytica
AssigneeBiosite Diagnostics, Inc.
InventorsGunars E. Valkirs, Joe Buechler, Jeff Gray
Life of this patent
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Abstract
This invention provides methods, reagents, and kits that are useful for diagnosing infection by E. histolytica. The methods are based on the discovery of binding agents, including recombinant polyclonal antibodies, that bind to the 29 kDa antigen of E. histolytica.
Claims15
A method of diagnosing infection of a mammal by an Entamoeba species, the method comprising:
- contacting a stool sample obtained from the mammal with a capture reagent which binds to a 29 kDa antigen of Entamoeba histolytica or Entamoeba dispar, wherein the capture reagent forms a complex with the 29 kDa antigen if the 29 kDa antigen is present in the stool sample;
- and
- detecting whether 29 kDa antigen is bound to the capture reagent, wherein the presence of 29 kDa antigen is indicative of Entamoeba infection of the mammal.
A kit for diagnosing infection of a mammal by an Entamoeba species, the kit comprising:
- a solid support upon which is immobilized a capture reagent which binds to a 29 kDa antigen of Entamoeba histolytica;
- and
- a detection reagent which binds to the 29 kDa antigen;
- wherein the capture reagent is selected from the group consisting of a) monoclonal antibody EH29.Ab. 13, and b) recombinant polyclonal antibody preparation EH29.Ab.32.PC.
A monoclonal antibody that specifically binds to 29 kDa antigen of E. histolytica, wherein the monoclonal antibody is EH29.Ab.13.
A recombinant polyclonal antibody preparation that specifically binds to 29 kDa antigen of E. histolytica, wherein the antibody preparation is EH29.Ab.32.PC.
Dependencies are inferred from the claim text.