Use of nucleic acids bound to carrier macromolecules
AssigneeAmdex A/S
InventorChristopher J. Stanley
Life of this patent
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Abstract
A nucleic acid primer is bound to a soluble carrier macromolecule, a multitude of primer molecules thus being carried by each carrier macromolecule, and hybridisation of the primer to a template followed by extension of the primer to replicate the template in complementary form is carried out as part of a PCR procedure or other amplification, or to form an extended primer of greater hybridization affinity. A second primer used in the amplification may also be bound to a carrier macromolecule.
Claims15
A method of detecting the presence of a first nucleic acid bound to a carrier macromolecule, comprising providing a second nucleic acid bound to a carrier macromolecule, wherein each carrier macromolecule is water soluble, contacting said nucleic acids under hybridization conditions, whereby a hybridization complex is formed, wherein the detection of the first nucleic acid is a function of the molecular mass of the hybridization complex.
A method of detecting the presence of a nucleic acid template sequence comprising replicating the template in complementary form, hybridizing to said template primer having a sequence complementary to a portion of said template, which primer is bound to a carrier macromolecule, and extending said primer to produce a replicated template bound to said carrier macromolecule and detecting the presence of said replicated template bound to the carrier macromolecule by providing a detection nucleic acid complementary in sequence to said replicated template which detection nucleic acid is bound to a carrier macromolecule, wherein each carrier macromolecule is water soluble, contacting said replicated template and detection nucleic acid under hybridization conditions, whereby a hybridization complex is formed, wherein the detection of the nucleic acid template sequence is a function of the molecular mass of the hybridization complex.
Dependencies are inferred from the claim text.