Reagents and methods useful for detecting diseases of the urinary tract

A method of detecting the presence of urinary tract diseases correlated with increased expression of SEQ ID Nos:

• 1-13 in an individual, said method comprising:
• (a) providing a test sample comprising nucleic acids from the individual and performing reverse transcription on said sample using at least one primer in order to produce cDNA;
• (b) amplifying the cDNA obtained from step (a) using oligonucleotides as sense and antisense primers to obtain an amplicon;
• and
• (c) detecting the increased expression of the mRNA of SEQ ID NO:s 1-13 by detection of increased amplicon amounts relative to wild type as an indication of urinary tract disease in the individual, wherein the oligonucleotides utilized in steps (a) and (b) consist of a sequence selected from the group consisting of SEQUENCE ID NOS 1 through 13, and exact complements thereof.

A method of detecting the presence of urinary tract diseases correlated with increased expression of SEQ ID Nos:

• 1-13 in an individual, said method comprising:
• (a) providing a test sample from the individual and contacting nucleic acids from the test sample with at least one oligonucleotide as a sense primer and with at least one oligonucleotide as an anti-sense primer and amplifying to obtain a first stage reaction product;
• (b) contacting said first stage reaction product with at least one other oligonucleotide to obtain a second stage reaction product, with the proviso that the other oligonucleotide is located 3′ to the oligonucleotides utilized in step (a) and is complementary to said first stage reaction product;
• and
• (c) detecting increased expression of mRNA of SEQ ID NO:s 1-13 by detection of increased amounts relative to wild type of said second stage reaction product as an indication of urinary tract disease in the individual, wherein the oligonucleotides utilized in steps (a) and (b) consist of sequence selected from the group consisting SEQUENCE ID NOS 1 throughl3, and exact complements thereof.

A method of detecting the presence of prostate cancer in an individual, said method comprising:

• (a) providing a test sample from the individual and performning reverse transcription on said sample using at least one primer in order to produce cDNA;
• (b) amplifying the cDNA obtained from step (a) using oligonucleotides as sense and antisense-primers to obtain amplicon;
• and
• (c) detecting increased expression of mRNA of SEQ ID NO:s 1-13 by detection of increased amounts relative to wild type of of said amplicon as an indication of prostate cancer in the individual, wherein the oligonucleotides utilized in steps (a) and (b) consist of sequence selected from the group consisting of SEQUENCE ID NOS. I through 13, and exact complements thereof.