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US 6,207,367 B1Granted
Process for genetic mapping for plant identification and breeding purposes
AssigneePioneer Hi-Bred International, Inc.
InventorsTimothy George Helentjaris, Gretchen Jane King
Life of this patent
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Abstract
The invention is in a process of genetic mapping for plant identification and breeding purposes. The process utilitzes restriction fragment technology and detection of polymorphisms to build up a genomic “fingerprint” for a variety or isolate. The “fingerprint” is then compared to “fingerprints” of other varieties or isolates to determine both the degree of relatedness and homozygosity for identification and breeding purposes.
Claims5
A process for comparing plants comprising the steps of:
- (A) providing a plurality of labelled DNA probes, wherein each probe of said plurality consists of a base sequence represented in low copy number in the genome of a first plant;
- (B) under hybridization conditions, bringing each of said DNA probes into contact with restriction fragments of genomic DNA of said first plant which are arrayed on a nucleic acid molecular hybridization matrix in a size-dependent manner, thereby to produce a plurality of nucleic acid molecular hybridization products;
- (C) from said plurality of nucleic acid molecular hybridization products, ascertaining a first pattern of hybridization of said DNA probes to said restriction fragments
- (D) repeating steps (B) and (C) with restriction fragments of the genomic DNA of a second plant, said restriction fragments of the genomic DNA of said second plant generated in the same way as said restriction fragments of the genomic DNA of said first plant, thereby ascertaining a second pattern of hybridization of said DNA probes to said restriction fragments of from said second plant;
- and
- (E) comparing said first pattern of hybridization to said second pattern of hybridization.
3 more elements
Dependencies are inferred from the claim text.