Method for inducing a systemic immune response to an HIV antigen
AssigneeBio-Sphere Technology, Inc.
InventorsJackie R. See, Darryl M. See
Life of this patent
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Abstract
A method is provided for inducing a systemic immune response to an antigen selected from inactivated HIV I and HIV II antigens in a mammal. The method comprises orally administering lyophilized multilaminar liposomes containing the antigen. The liposomes have a size of from 20 nm to 20 microns. The antigen-containing liposomes are absorbed in the Peyer's patches of the gut. Sufficient antigen-containing liposomes are taken up by macrophages in the Peyer's patches to induce a systemic immune response to the antigen.
Claims51
A method for stimulating a systemic immune response to an antigen selected from the group consisting of inactivated HIV I and HIV II antigens and combinations thereof in a mammal comprising:
- providing a lipsomal preparation comprising lyophilized liposomes containing at least one antigen selected from the group consisting of inactivated HIV I and HIV II antigens, wherein the liposomes have at least three different sizes and consist essentially of:
- at least 5% by volume, based on the total volume of liposomes in the preparation, small liposomes having a size, before lyophilization, of from about 20 nm to about 1 micron,
- at least 5% by volume, based on the total volume of liposomes in the preparation, medium liposomes having a size, before lyophilization, of from about 1 micron to about 3 microns, and
- at least 5% by volume, based on the total volume of liposomes in the preparation, large liposomes having a size, before lyophilization, of from about 3 microns to about 20 microns;
- and
- orally administering an effective amount of the liposomal preparation to a mammal,
- whereby sufficient antigen containing liposomes are absorbed in the Peyer's patches of the gut of the mammal and are taken up by macrophages in the Peyer's patches to stimulate a systemic immune response.
A preparation for oral administration to a mammal capable of stimulating a systemic immune response to at least one antigen selected from the group consisting of inactivated HIV I and HIV II antigens, said preparation comprising an effective amount of lyophilized antigen-containing liposomes, wherein the liposomes have at least three different sizes, before lyophilization, and consist essentially of:
- at least 5% by volume, based on the total volume of liposomes in the preparation, small liposomes having a size, before lyophilization, of from about 20 nm to about 1 micron,
- at least 5% by volume, based on the total volume of liposomes in the preparation, medium liposomes having a size, before lyophilization, of from about 1 micron to about 3 microns, and
- at least 5% by volume, based on the total volume of liposomes in the preparation, large liposomes having a size, before lyphilization, of from about 3 microns to about 20 microns.
Dependencies are inferred from the claim text.