Fluorescent imaging method of saccharide uptake activity of living tissue
AssigneeTokyo University of Agriculture and Technology
InventorHideaki Matsuoka
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Abstract
A fluorescent imaging method of the changes in viability of living bodies wherein the living bodies are provided with a chemical or physical exterior stimulation and information on the changes in viability of cells caused by the stimulation is obtained by means of a fluorescent imaging method of the saccharide uptake activity by contacting or administering to the living bodies an aqueous solution of a fluorescent saccharide compound which includes at its specific position a saccharide molecule chemically combined with a fluorescent pigment molecule.
Claims7
A method of detecting the saccharide uptake activity of living tissue containing living bodies having biological activity comprising the steps of:
- forming an aqueous solution of a fluorescent saccharide compound comprising a saccharide molecule chemically combined at a specific position thereof with a fluorescent pigment molecule having a molecular weight no greater than 300;
- contacting said tissue with said aqueous solution of the fluorescent saccharide compound such that said fluorescent saccharide compound is incorporated therein;
- and analyzing the tissue having the fluorescent saccharide compound incorporated therein with a fluorescence detection device.
Dependencies are inferred from the claim text.