Devices and methods for applying a mixture of two or more liquid components to form a biomaterial

A method for spray application of a fibrin sealant to a desired site by the simultaneous application of gas and at least two liquids which form said biomaterial upon mixing, which method comprises the steps of:

• a) using an application system comprising separate sources of said gas and said liquids in fluid communication with a longitudinal spray tip via tubing, said spray tip having a flat outlet surface normal to said longitudinal direction, said tubing being connected to said sources and said tip, wherein said sources of gas and liquids are remote from said spray tip such that a user does not need to manually hold said sources while applying said fibrin sealant, wherein said tubing has a discrete channel for each of said liquids and said gas, each channel terminating at said flat outlet surface, and wherein said liquids include a fibrin monomer solution and a polymerizing liquid capable of polymerizing said fibrin monomer solution;
• b) applying said liquids by the simultaneous transferring of said liquids and gas from said sources through said tubing to, and out of, said spray tip so that the liquids are mixed as they are applied to the desired site;
• and
• c) maintaining the gas flow below 1.25 liters/minute while maintaining the ratio of the gas flow to the total flow of the two or more liquids between 150:1 and 1500:1.