Pesticidal toxins and nucleotide sequences which encode these toxins
AssigneeMycogen Corporation
InventorsJerald S. Feitelson, H. Ernest Schnepf, Kenneth E. Narva, Brian A. Stockhoff, James Schmeits, David Loewer, Charles Joseph Dullum, Judy Muller-Cohn, Lisa M. Stamp
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Abstract
Disclosed and claimed are novel Bacillus thuringiensis isolates, pesticidal toxins, genes, and nucleotide probes and primers for the identification of genes encoding toxins active against pests. The primers are useful in PCR techniques to produce gene fragments which are characteristic of genes encoding these toxins. The subject invention provides entirely new families of toxins from Bacillus isolates.
Claims48
An isolated polynucleotide which encodes a pesticidally active protein wherein said polynucleotide hybridizes under conditions of 0.1× SSPE at 65° C. with the full complement of a nucleotide sequence selected from the group consisting of SEQ ID NO. 18, SEQ ID NO. 22, SEQ ID NO. 26, and SEQ ID NO. 47.
An isolated polynucleotide which encodes a pesticidally active protein wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO. 10.
An isolated polynucleotide which encodes a pesticidlly active protein wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO. 12.
An isolated polynucleotide which encodes a pesticidally active protein wherein said protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO. 19, SEQ ID NO. 23, SEQ ID NO. 27, and SEQ ID NO. 44.
A recombinant host comprising an isolated polynucleotide which encodes a pesticidally active protein, wherein said host is a plant, and wherein said polynucleotide hybridizes under conditions of 0.1× SSPE at 65° C. with the full complement of a nucleotide sequence selected from the group consisting of SEQ ID NO. 18, SEQ ID NO. 22, SEQ ID NO. 26, and SEQ ID NO. 47.
A recombinant host comprising an isolated polynucleotide which encodes a pesticidally active protein, wherein said host is a plant, and wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO. 10.
A recombinant host comprising an isolated polynucleotide which encodes a pesticidally active protein, wherein said host is a plant, and wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO. 12.
A recombinant host comprising a polynucleotide sequence which encodes a pesticidally active protein, wherein said host is a plant, and wherein said protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO. 19, SEQ ID NO. 23, SEQ ID NO. 27, and SEQ ID NO. 44.
A recombinant host comprising an isolated polynucleotide which encodes a pesticidally active protein, wherein said host is a plant cell, and wherein said polynucleotide hybridizes under conditions of 0.1× SSPE at 65° C. with the full complement of a nucleotide sequence selected from the group consisting of SEQ ID NO:18, SEQ ID NO:22, SEQ ID NO:26, and SEQ ID NO:47.
A recombinant host comprising an isolated polynucleotide which encodes a pesticidally active protein, wherein said host is a microbial cell, and wherein said polynucleotide hybridizes under conditions of 0.1× SSPE at 65° C. with the full complement of a nucleotide sequence selected from the group consisting of SEQ ID NO:18, SEQ ID NO:22, SEQ ID NO:26, and SEQ ID NO:47.
A recombinant host comprising an isolated polynucleotide which encodes a pesticidally active protein, wherein said host is a plant cell, and wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO:10.
A recombinant host comprising an isolated polynucleotide which encodes a pesticidally active protein, wherein said host is a microbial cell, and wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO:10.
A recombinant host comprising an isolated polynucleotide which encodes a pesticidally active protein, wherein said host is a plant cell, and wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO:12.
A recombinant host comprising an isolated polynucleotide which encodes a pesticidally active protein, wherein said host is a microbial cell, and wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO:12.
A recombinant host comprising a polynucleotide sequence which encodes a pesticidally active protein, wherein said host is a plant cell, and wherein said protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:19, SEQ ID NO:23, SEQ ID NO:27, and SEQ ID NO:44.
A recombinant host comprising a polynucleotide sequence which encodes a pesticidally active protein, wherein said host is a microbial cell, and wherein said protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:19, SEQ ID NO:23, SEQ ID NO:27, and SEQ ID NO:44.
Dependencies are inferred from the claim text.