Microinjection methods to transform arthropods with exogenous DNA
AssigneesUniversity of Florida, The Regents of the University of California
InventorsMarjorie A. Hoy, James K. Presnail
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Abstract
This invention relates to methods for transforming arthropods with exogenous DNA. The methods are useful for arthropods that have not previously been amenable to DNA-mediated transformation, and also provide a simpler and more efficient means of transforming arthropods than have previously been described. The method involves microinjecting a nucleic acid sequence encoding a desired trait into the reproductive tract of a female arthropod before oviposition.
Claims3
A method for producing a transformed insect, the method comprising microinjecting a nucleic acid sequence encoding a desired trait into the reproductive tract of a female insect before oviposition, such that a transformed ovum results, and said transformed ovum is permitted to develop into a transformed insect.
Dependencies are inferred from the claim text.