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AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsTomoyuki Nishimoto, Michio Kubota, Hiroto Chaen, Toshio Miyake
Estimating the term…
A kojibiose phosphorylase which hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and β-D-glucose-1-phosphoric acid, forms kojibiose and an inorganic phosphoric acid from β-D-glucose-1-phosphoric acid, and catalyzes the transfer reaction of glucosyl group to other saccharides using β-D-glucose-1-phosphoric acid as a saccharide donor. The enzyme is obtainable from natural sources such as microorganisms of the genus Thermoanaerobium, and obtainable by recombinant technology. Kojibiose phosphorylase is used to transfer glucose from glucose-1-phosphate to acceptor saccharides to produce α-D-glucosyl-1(1→5)-L-sorbose, kojibiosylglucose, kojibiosylglucoside and kojibiosylfructoside.
A saccharide composition comprising a glucosyl-transferred saccharide producible by
A glucosyl-transferred saccharide selected from the group consisting of glucosylsorbose represented by α-D-glucosyl-(1→5)-L-sorbose, and kojibiosylfructoside represented by α-D-glucosyl-(1→2)-α-D-glucosyl-(1→2)-β-D-fructoside.
A saccharide composition comprising a glucosyl- transferred saccharide produced by contacting β-D-glucose-1-phosphoric acid and/or its salt and other saccharide with a kojibiose phosphorylase which catalyzes the phosphorolysis of kojibiose in the presence of an inorganic phosphoric acid and/or its salt to form D-glucose and β-D-glucose-1-phosphoric acid and/or its salt, wherein the glucosyl-transferred saccharide is a member selected from the group consisting of α-D-glucosyl-(1→5)-L-sorbose, kojibiosylglucose, kojibiosylglucoside, kojibiosylfructoside and mixtures thereof.
Dependencies are inferred from the claim text.