Specific binding agents
AssigneeUnilever Patent Holdings B.V.
InventorMartine Elisa Verhoeyen
Life of this patent
Estimating the term…
Abstract
A reshaped human antibody or reshaped human antibody fragment having specificity for human polymorphic epithelial mucin (PEM) is produced by transferring the complementarity determining regions (CDRS) from a murine anti-HMFG hybridoma cell line HMFG1 into a human antibody variable region framework. The reshaped molecule can be used in the treatment or diagnosis of cancer.
Claims21
An antibody or antibody fragment having specificity for human polymorphic endothelial mucia (PEM), said antibody or fragment incorporating a heavy chain variable region comprising the amino acid sequence represented by SEQ ID NO:60 and a light chain variable region comprising the amino acid sequence represented by SEQ ID NO:62.
A stable host cell line comprising a nucleic acid molecule represented by SEQ ID NO:59.
A stable host cell line comprising a nucleic acid molecule represented by SEQ ID NO:61.
A stable host cell line comprising a nucleic acid molecule that encodes amino acid sequence SEQ ID NO:60.
A stable host cell line comprising a nucleic acid molecule that encodes amino acid sequence SEQ ID NO:62.
Plasmid pSVgpt-HuVG-1-HuIgG1, deposited as NCTC 12411.
Plasmid pSVneo-HuVkHMG1-HuCk, deposited as NCTC 1241.
A method of producing an antibody by expressing the plasmids pSVgpt-HuVHHMFG-1-HuIgG1 deposited as NCTC 12411, and pSVneo-HuVkHMFG1-HuCk, deposited as NCTC 12412, thereby producing said antibody.
E. coli NCTC 12411.
E. coli NCTC 12412.
A DNA molecule encoding an antibody heavy-chain variable region having specificity for HMFG, as contained in E. coli NCTC 12411.
A DNA molecule encoding an antibody light-chain variable region having specificity for HMFG, as contained in E. coli NCTC 12412.
An antibody or antigen binding fragment thereof, comprising at least one light chain variable region comprising the sequence represented by SEQ ID NO:60 and one heavy chain variable region comprising the sequence represented by SEQ ID NO:62.
Dependencies are inferred from the claim text.