Polynucleotides and the proteins encoded thereby, suitable for controlling lamellicorn beetles
AssigneeSyngenta Participations AG
InventorsWolfgang Schnetter, Lutz Krieger, Jiambing Zhang
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Abstract
The invention relates to isolated polynucleotides and the proteins encoded thereby, and to their use in controlling lamellicorn beetles (Scarabaeidae). In addition, the invention relates to a method of producing those proteins. The polynucleotides of the invention encode proteins that are identical to or at least related to the crystal proteins characteristic of Bacillus popilliae and that are suitable for the inhibition of the feeding activity and/or for the destruction of adult and/or larval scarabaeids, especially Melolontha species and species closely related thereto.
Claims17
An isolated nucleic acid molecule comprising a nucleotide sequence that encodes a protein having insecticidal activity against scarabaeids, said protein comprising an amino acid sequence that is the translation product of a nucleic acid sequence whose complement hybridizes to SEQ ID NO:1 under hybridization conditions of 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 50° C., or under hybridization conditions of 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 65° C.
An isolated nucleic acid molecule comprising a nucleotide sequence that encodes a protein having insecticidal activity against scarabaeids, wherein said nucleotide sequence has a complement that hybridizes to SEQ ID NO:1 under hybridization conditions of 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 50° C., or under hybridization conditions of 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 65° C.
Dependencies are inferred from the claim text.