Producing diastereomers of 4-hydroxyproline using 4-hp epimerase from Serratia marcescens or Acinetobacter baumanni
AssigneeE. I. Du Pont de Nemours and Company
InventorsRobert Dicosimo, Susan K. Fager, John E. Gavagan
Life of this patent
Estimating the term…
Abstract
The present invention relates to two novel microorganisms, Acinetobacter baumanni WT-50/50-4A (ATCC 202144) and Serratia marcescens WT-L-4A1(RED) (ATCC 202145) characterized by 4-hydroxyproline epimerase activity. The compounds trans-4-hydroxy-L-proline (THLP) or cis-4-hydroxy-D-proline (CHDP) are converted with this epimerase activity to a mixture of THLP and CHDP. Additionally, the compounds trans-4-hydroxy-D-proline (THDP) or cis-4-hydroxy-L-proline (CHLP) are converted with this epimerase activity to a mixture of THDP and CHLP.
Claims6
A process to produce diastereomers of 4-hydroxyproline comprising:
- (a) contacting, in an aqueous reaction mixture, at least one starting material selected from the group consisting of trans-4-hydroxy-L-proline and cis-4-hydroxy-D-proline with an enzyme catalyst in a form selected from the group consisting of whole cells, cell extracts, partially purified enzyme or purified enzyme, the enzyme catalyst characterized by 4-hydroxyproline epimerase activity and derived from Acinetobacter baumanni WT-50/50-4A (ATCC 202144) or Serratia marcescens WT-L-4A1 (RED) (ATCC 202145);
- and
- (b) isolating trans-4-hydroxy-L-proline and cis-4-hydroxy-D-proline from the reaction mixture.
A process to produce diastereomers of 4-hydroxyproline comprising:
- (a) contacting, in an aqueous reaction mixture, at least one starting material selected from the group consisting of trans-4-hydroxy-D-proline and cis-4-hydroxy-L-proline, with an enzyme catalyst in a form selected from the group consisting of whole cells, cell extract, partially purified enzyme or purified enzyme, the enzyme catalyst characterized by 4-hydroxyproline epimerase activity and derived from Acinetobacter baumanni WT-50/50-4A (ATCC 202144) or Serratia marcescens WT-L-4A1 (RED) (ATCC 202145);
- and
- (b) isolating trans-4-hydroxy-D-proline and cis-4-hydroxy-L-proline from the reaction mixture.
A biologically pure culture of Acinetobacter baumanni WT-50/50-4A designated as ATCC Accession Number 202144.
A biologically pure culture of Serratia marcescens WT-L-4A (RED) designated as ATCC Accession Number 202145.
Dependencies are inferred from the claim text.