Plant isocitrate dehydrogenase homologs
AssigneeE. I. Du Pont de Nemours and Company
InventorsStephen M. Allen, Saverio Carl Falco
Life of this patent
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Abstract
This invention relates to an isolated nucleic acid fragment encoding an isocitrate dehydrogenase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the isocitrate dehydrogenase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the isocitrate dehydrogenase in a transformed host cell.
Claims19
An isolated polynucleotide selected from the group consisting of:
- (a) SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 or SEQ ID NO:7;
- (b) a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8;
- and
- (c) a nucleic acid sequence that is complementary to (a) or (b).
A method of obtaining an isolated polynucleotide encoding an isocitrate dehydrogenase polypeptide comprising:
- (a) synthesizing an oligonucleotide primer corresponding to at least 30 contiguous nucleotides of the isolated polynucleotide set forth in any of SEQ ID NOs:1, 3, 5, and 7;
- and
- (b) amplifying a cDNA insert present in a cloning vector using the oligonucleotide primer of step (a)
- wherein the amplified isolated polynucleotide encodes an isocitrate dehydrogenase polypeptide.
An isolated polynucleotide comprising a nucleic acid selected from the group consisting of:
- (a) a polynucleotide that is at least 90% indentical by the Jotun Hein method of alignment to an isolated polynucleotide encoding the isocitrate dehydrogenase polypeptide set forth in a nucleic acid selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO:
- 6 and SEQ ID NO:
- 8, wherein the isolated polynucleotide encodes an isocitrate dehydrogenase polypeptide;
- and
- (b) polynucleotide that is complementary to the polynucleotide of (a).
Dependencies are inferred from the claim text.