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US 6,204,028 B1Granted
Methods for identifying antiviral agents against human herpesviruses
AssigneeThe Trustees of the University of Pennsylvania
InventorsRobert P. Ricciardi, Kai Lin
Life of this patent
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Abstract
A method of screening compounds for antiviral activity against a selected human herpesvirus by measuring inhibition of DNA synthesis by DNA polymerase and processivity factor of the selected human herpesvirus in the presence of the compound is provided.
Claims1
A method of screening compounds in vitro for antiviral activity against a human herpesvirus 6 (HHV-6) or a human herpesvirus 8 (HHV-8) comprising:
- (a) selecting the human herpesvirus to be a target for antiviral activity wherein said selected human herpesvirus is selected from the group consisting of HHV-6 or HHV-8;
- (b) immobilizing a DNA of the selected human herpesvirus to a solid support;
- (c) contacting the DNA of the human herpesvirus with a corresponding human herpesvirus DNA polymerase and a corresponding human herpesvirus processivity factor;
- (d) detecting newly synthesized DNA in the presence and absence of a compound to be tested for antiviral activity wherein inhibition of DNA synthesis in the presence of the compound is indicative of antiviral activity against the human herpesvirus;
- and
- (e) repeating steps (a) through (d) in the presence and absence of the DNA polymerase and the combination of the DNA polymerase and the DNA processivity factor.
3 more elements
Dependencies are inferred from the claim text.