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US 6,204,018 B1Granted
66 kDa antigen from Borrelia
AssigneeSymbicom Aktiebolag
InventorsSven Bergstöm, Alan George Barbour
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AssigneeSymbicom Aktiebolag
InventorsSven Bergstöm, Alan George Barbour
Estimating the term…
Nucleic acid fragments are disclosed which encode a polypeptide antigen reactive with antisera from rabbits immunised with a 66 kDa protein from Borrelia garinii IP90. The presence of nucleic acid fragments encoding such a polypeptide antigen as well as the presence of the polypeptide antigen have been demonstrated in three strains of B. burgdorferi sensu lato, but are substantialle absent from at least 95% of randomly selected B. hermsii, B. crocidurae, B. anserina, and B. hispanica. The encoded polypeptide is surface exposed on the bacterial surface, it is highly conserved, and is thus potentially useful as a vaccine agent and as a diagnostic agent in the diagnosis of infections with B. burgdorferi as are the characteristic nucleic acid fragments of the invention. Also disclosed are methods of producing the polypeptide antigen according to the invention as are antibodies directed against the antigen.
A vector comprising an isolated DNA molecule which comprises a nucleotide sequence encoding a 66 KDa Borrelia protein as set forth in SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7 or SEQ ID NO:13.
A vector comprising an isolated DNA molecule which comprises a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, or SEQ ID NO:14.
A vector comprising an isolated DNA molecule encoding a 66 KDa membrane protein of Borrelia burgdorferi sensu stricto 31, Borrelia afzelli ACAI or Borrelia garinii IP90, said 66 KDa membrane protein being immunoenically active.
A vector comprising an isolated DNA molecule which comprises a nucleotide sequence encoding a 66 KDa membrane protein of Borrelia afzelli ACAI, said 66 KDa membrane protein being immunogenically active.
A vector comprising an isolated DNA molecule which comprises a nucleotide sequence encoding a 66 KDa membrane protein of Borrelia garinii IP90, said 66 KDa membrane protein being immunogenically active.
A vector comprising an isolated DNA molecule encoding a polypeptide having an amino acid sequence selected from the group of amino acid sequences of 175 to 190, 285 to 305, 365 to 385 and 465 to 490 from SEQ ID NOS:4, 6, 8 and 14.
Dependencies are inferred from the claim text.