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AssigneeRoche Vitamins Inc.
InventorsKarsten Hellmuth, Rual Lopez-Ulibarri, Anne Françoise Mayer, Heinrich Winfried Schlieker, Adolphus van Loon
Estimating the term…
A method for producing a protein by culturing a cell capable of expressing the protein and which contains a methylotrophic yeast promoter for an enzyme of the methanol metabolic pathway controlling expression of the protein, in a fermentative batch process comprising a batch phase and a feeding phase, under conditions such that dissolved oxygen is continually present in the culture medium throughout the process, and about 1% to about 100% of the carbon source during the feeding phase is a sugar or sugar polymer, which sugar or sugar polymer is provided in such an amount that the sugar or sugar polymer is continually depleted by the cell and therefore is substantially undetectable in the culture medium; and isolating the protein on completion of the feeding phase.
A method for producing a desired protein which comprises culturing a cell capable of expressing the protein comprising a nucleic acid sequence encoding the desired protein operably linked to a methylotrophic yeast promoter having an activity for an enzyme of the methanol metabolic pathway and controlling expression of the desired protein, in a fermentative batch process comprising a batch phase and a feeding phase under conditions such that dissolved oxygen is continually present in the culture medium throughout the process, and about 1% to about 100% of the total carbon source present in the feeding phase is a repressive sugar or a repressive sugar polymer, which is provided in such an amount that the repressive sugar or repressive sugar polymer is continually depleted by the cell and therefore substantially undetectable in the culture medium;
Dependencies are inferred from the claim text.