Human receptor tyrosine kinase, KDR
AssigneeMerck & Co., Inc.
InventorsRichard L. Kendall, Xianzhi Mao, Kenneth A. Thomas, Andrew Tebben
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Abstract
An isolated nucleic acid molecule encoding a novel human receptor type tyrosine kinase gene, KDR, is disclosed. The isolation of this KDR cDNA sequence results in disclosure of purified forms of human KDR protein, recombinant vectors and recombinant hosts which express human KDR.
Claims26
A purified DNA molecule which consists of the nucleotide sequence as set forth in SEQ ID No:1.
A process for the expression of a human KDR protein in a recombinant host cell, comprising:
- (a) transfecting an expression vector which encodes a human KDR protein as set forth in SEQ ID NO:2 into a suitable host cell;
- and,
- (b) culturing the host cells of step (a) under conditions which allow expression of the human KDR protein from the expression vector.
A purified nucleic acid molecule encoding an intracellular portion of a human KDR protein which comprises from about amino acid 790 to about amino acid 1356 as set forth in SEQ ID NO:
- 2, wherein position 848 is a valine residue.
A purified nucleic acid molecule encoding a soluble KDR fusion protein which comprises from about amino acid 790 to about amino acid 1356 of human KDR as set forth in SEQ ID NO:
- 2, wherein position 848 is a valine residue.
A purified DNA molecule encoding a human KDR protein wherein said protein consists of the amino acid sequence as set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.