Nucleic acids encoding mutant recombinant hemoglobins containing heme pocket mutations
AssigneeBaxter Biotech Technology Sarl
InventorsJohn S. Olson, Antony J. Mathews, Jacqueline F. Aitken, Kyoshi Nagai
Life of this patent
Estimating the term…
Abstract
This invention relates to recombinant hemoglobins containing mutations in or near the heme pocket of the hemoglobin molecule. This invention particularly relates to recombinant hemoglobins that have altered geometry or polarity around the distal portion of the heme pocket.
Claims24
An isolated DNA sequence encoding a mutant recombinant globin protein of a hemoglobin molecule comprising a recombinant globin with one or more mutations of any amino acid of a heme pocket of said recombinant globin, wherein said mutation is not B10(Leu→Gln) or CD3(Ser→Arg) of the β-globin chain.
A mutant recombinant hemoglobin has one or more amino acid mutations selected from the group consisting of B10Leu , B13 Met, CE1 Phe, CE3 His, E7 His, CE4 Phe, E11 Val, E10 Lys, G8 Leu, E14 Ala, and G12 Leu for alpha globin and B10 Leu, B13 Leu, CD1 Phe, CD3 Ser, E7 His, CD4 Phe, E11 Val, E10 Lys, G8 Leu, and E14 Ala for beta globin.
A mutant recombinant hemoglobin has one or more of the mutations selected from the group consisting of Leu-B10→Phe, Val-E11→Phe and His-E7→Gln for alpha and/or beta globin.
A mutant recombinant hemoglobin has one or more of the mutations selected from the group consisting of Leu-B10→Trp, Leu-B10→Phe, Leu-B10→Val, Leu-B10→Ile, Leu-B10→Ala, His-E7→Leu, His-E7→Trp, Val-E11→Phe, and Val-E11→Trp.
A non-naturally occurring nucleotide sequence encoding a mutant recombinant globin protein of a hemoglobin molecule comprising a recombinant globin with one or more mutations of any amino acid of a heme pocket of said recombinant globin.
Dependencies are inferred from the claim text.