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US 6,204,008 B1Granted
Bioprocess for production of dipeptide based compounds
AssigneeMerck & Co., Inc.
InventorsW. Scott Borneman, Anil Goyal, Michael J. Conder, Victor A. Vinci
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Abstract
Bioprocesses are disclosed for the production of compounds which can be produced from a dipeptide intermediate. The process comprises production of a recombinant polypeptide which contains the dipeptide intermediate. The dipeptide intermediate is further processed to ultimately provide the finished product.
Claims13
A process for producing an Ala-Pro dipeptide, which comprises:
- (a) transfecting a DNA expression vector into suitable host cells wherein said DNA expression vector comprises a DNA molecule which encodes an oligopeptide or polypeptide containing multiple direct repeats of said Ala-Pro dipeptide;
- (b) culturing said host cells under conditions suitable for expression of said oligopeptide or polypeptide from said DNA expression vector;
- (c) cleaving said expressed oligopeptide or polypeptide with an enzyme capable of cleaving said Ala-Pro dipeptides from each other, thereby releasing said Ala-Pro dipeptides, wherein said enzyme is selected as a combination of at least an exo- and an endopeptidase selected from the group consisting of:
- factor X, Lactobacillus sp. prolypeptidase, Lactobacillus helveticus prolypeptidase, Flavobacterium meningosepticum prolypeptidase, and Xanthomonas maltophilia prolypeptidase;
- and,
- (d) isolating and purifying said Ala-Pro dipeptides.
3 more elements
A process for producing a Lys-Pro dipeptide, which comprises:
- (e) transfecting a DNA expression vector into suitable host cells wherein said DNA expression vector comprises a DNA molecule which encodes an oligopeptide or polypeptide containing multiple direct repeats of said Lys-Pro dipeptide;
- (f) culturing said host cells under conditions suitable for expression of said oligopeptide or polypeptide from said DNA expression vector;
- (g) cleaving said expressed oligopeptide or polypeptide with an enzyme capable of cleaving said Lys-Pro dipeptides from each other, thereby releasing said Lys-Pro dipeptides, wherein said enzyme is selected as a combination of at least an exo- and an endopeptidase selected from the group consisting of:
- factor X, Lactobacillus sp. prolypeptidase, Lactobacillus helveticus prolypeptidase, Flavobacterium meningosepticum prolypeptidase, and Xanthomonas maltophilia prolypeptidase;
- and,
- (h) isolating and purifying said Lys-Pro dipeptides.
3 more elements
Dependencies are inferred from the claim text.