Methods for the diagnosis of feline infectious anemia
AssigneeSynbiotics Corporation
InventorsJ. Kevin Steele, David L. Telford, John A. Cutting
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Abstract
The claimed invention is a method for determining whether a mammal is infected with Haemobartonella felis or for inducing an immune response against Haemobartonella felis using a polypeptide expressed by Mycoplasma. Preferably, the polypeptide is expressed by Mycoplasma gallisepticum. In a preferred embodiment the polypeptide is the pMGA protein expressed by the strain of Mycoplasma gallisepticum having ATCC deposit number 19610.
Claims5
A method for detecting the presence of Haemobartonella felis in a mammal comprising the steps of:
- obtaining a sample of a bodily fluid from said mammal;
- contacting said sample with an antigen from Mycoplasma, said antigen from Mycoplasma being an antigen which is recognized by antibodies in serum, plasma, or blood from mammals infected with Haemobartonella felis but not by antibodies in serum, plasma, or blood from mammals which are not infected with Haemobartonella felis;
- and
- determining whether said antibodies in said sample bind to said antigen from Mycoplasma.
A method for detecting the presence of Haemobartonella felis in a mammal comprising the steps of:
- growing a culture of Mycoplasma cells, said Mycoplasma cells expressing an antigen which is recognized by antibodies in serum, plasma, or blood from mammals infected with Haemobartonella felis but not by antibodies in serum, plasma, or blood from mammals which are not infected with Haemobartonella felis;
- fixing said antigen to a solid support;
- contacting said solid support with a sample of a bodily fluid from said mammals;
- and
- determining whether antibodies in said sample bind to said antigen from Mycoplasma.
Dependencies are inferred from the claim text.