Nucleic acid primers and probes for detecting tumor cells
AssigneeAbbott Laboratories
InventorsEdward N. Granados, Christi Powell Scheffel
Life of this patent
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Abstract
Nucleic acid sequences are provided that are useful as amplification primers and hybridization probes for detecting CK19 target sequence in a test sample. The primers and probes can be employed in amplification based methods for detecting the presence of CK19 sequences in a test sample. The amplification product can be detected with the assistance of microparticle capture techniques.
Claims7
A composition of matter comprising an oligonucleotide which consists of SEQ ID NO:
- 2 and an oligonucleotide which consists of SEQ ID NO:
- 3.
A composition of matter for detecting a CK19 target sequence comprising an oligonucleotide which consists of SEQ ID NO:
- 2, an oligonucleotide which consists of SEQ ID NO:
- 3, and a sequence selected from the group consisting of SEQ ID NO 4, SEQ ID NO 5, SEQ ID NO 6, and SEQ ID NO 7.
A kit for amplifying a CK19 target sequence comprising:
- (a) an oligonucleotide which consists of SEQ ID NO:
- 2 and an oligonucleotide which consists of SEQ ID NO:
- 3;
- and
- (b) amplification reagents.
Dependencies are inferred from the claim text.