Capture of single stranded nucleic acids
Assigneedu Vergier Ltd.
InventorsMartin J. Davies, Ian James Bruce
Life of this patent
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Abstract
A solid phase substrate derivatized with single stranded nucleic acids having random sequences of nucleotide bases and related method of capturing one or more single stranded nucleic acids by contacting with the solid phase substrate wherein the single stranded nucleic acids anneal to the nucleic acids of the substrate to result in the captured sequences.
Claims13
A method of assaying labeled double stranded amplification products in a fluid medium expected to further contain labeled single stranded nucleic acids including primers, wherein the amplification product labels and the single stranded nucleic acid labels are the same, comprising:
- (a) contacting the fluid medium with a solid phase substrate derivatized with single stranded nucleic acids having random sequences;
- (b) capturing the labeled single stranded nucleic acids by hybridizing to the random sequence nucleic acids on the solid phase substrate;
- (c) removing the solid phase substrate with the captured labeled single stranded nucleic acids;
- (d) assaying the label remaining in the fluid medium to thereby assay the labeled double stranded amplification products.
Dependencies are inferred from the claim text.