Chemiluminescent immunoassay for detection of antibodies to various viruses
AssigneeAbbott Laboratories
InventorsDinesh O. Shah, James P. Mackowiak, Natalie Dubovoy
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Abstract
The subject invention relates generally to immunoassays for detection of antibodies by use chemiluminescent compounds. More particularly, the subject invention relates to chemiluminescent immunoassays to detect antibodies wherein a precomplex mixture is created and a two-step assay is performed resulting in a greater signal.
Claims30
A method for determining the presence of a Chagas Disease analyte in a test sample by specific amplification of a chemiluminescent signal generated from a heterogeneous immunoasssay comprising the steps of:
- a. incubating a test sample containing a Chagas Disease analyte with an analyte-specific binding pair member for a time and under conditions sufficient to form analyte/analyte specific-binding member pair complexes;
- b. contacting the analyte/analyte-specific binding member pair complexes with a precomplex wherein said precomplex comprises 1) a probe comprising an enhancer compound attached to an analyte-specific binding member different from said analyte-specific binding member of step (a) and 2) a conjugate comprising a chemiluminescent signal generating compound attached to an enhancer-specific binding member, and incubating said resulting mixture for a time and conditions sufficient to form analyte/analyte-specific binding member pair/precomplex complexes
- wherein said chemiluminescent signal generating compound is an acridinium compound or a derivative thereof;
- c. separating said resulting analyte/analyte-specific binding member pair/precomplex complexes of step b from free, unbound precomplexes;
- and
- d. determining the presence of said Chagas Disease analyte in said test sample by measuring the detectable signal.
A kit for detecting a Chagas Disease analyte, comprising a Chagas disease analyte-specific binding pair member;
- and
- a single container containing a precomplex reagent wherein said precomplex reagent comprises 1) a probe which comprises an enhancer compound and 2) a conjugate which comprises a chemiluminescent signal-generating compound, wherein said chemiluminescent signal-generating compound is an acridinium compound.
A method for determining the presence of an HTLV analyte in a test sample by specific amplification of a chemiluminescent signal generated from a heterogeneous immunoasssay comprising the steps of:
- a. incubating a test sample containing an HTLV analyte with a biotinylated lysate comprising an analyte-specific binding pair member for a time and under conditions sufficient to form analyte/analyte specific-binding member pair complexes;
- b. contacting the analyte/analyte-specific binding member pair complexes with a precomplex wherein said precomplex comprises 1) a probe comprising an enhancer compound attached to an analyte-specific binding member which may be either the same or different from said analyte-specific binding member of step (a) and 2) a conjugate comprising a chemiluminescent signal generating compound attached to an enhancer-specific binding member, and incubating said resulting mixture for a time and conditions sufficient to form analyte/analyte-specific binding member pair/precomplex complexes
- wherein said chemiluminescent signal generating compound is an acridinium compound or a derivative thereof;
- c. separating said resulting analyte/analyte-specific binding member pair/precomplex complexes of step b from free, unbound precomplexes;
- and
- d. determining the presence of said HTLV analyte in said test sample by measuring the detectable signal.
A kit for detecting a HTLV analyte, comprising a HTLV analyte-specific binding pair member;
- and
- a single container containing a precomplex reagent wherein said precomplex reagent comprises 1) a probe which comprises an enhancer compound and 2) a conjugate which comprises a chemiluminescent signal-generating compound, wherein said chemiluminescent signal-generating compound is an acridinium compound.
A method for determining the presence of an HIV analyte in a test sample by specific amplification of a chemiluminescent signal generated from a heterogeneous immunoasssay comprising the steps of:
- a. incubating a test sample containing an HIV analyte with a biotinylated analyte-specific binding pair member for a time and under conditions sufficient to form analyte/analyte specific-binding member pair complexes;
- b. contacting the analyte/analyte-specific binding member pair complexes with a precomplex wherein said precomplex comprises 1) a probe comprising an enhancer compound attached to an analyte-specific binding member which may be either the same or different from said analyte-specific binding member of step (a) and 2) a conjugate comprising a chemiluminescent signal generating compound attached to an enhancer-specific binding member, and incubating said resulting mixture for a time and conditions sufficient to form analyte/analyte-specific binding member pair/precomplex complexes
- wherein said chemiluminescent signal generating compound is an acridinium compound or a derivative thereof;
- c. separating said resulting analyte/analyte-specific binding member pair/precomplex complexes of step b from free, unbound precomplexes;
- and
- d. determining the presence of said HIV analyte in said test sample by measuring the detectable signal.
A kit for detecting a HIV analyte, comprising a HIV analyte-specific binding pair member;
- and
- a single container containing a precomplex reagent wherein said precomplex reagent comprises 1) a probe which comprises an enhancer compound and 2) a conjugate which comprises a chemiluminescent signal-generating compound, wherein said chemiluminescent signal-generating compound is an acridinium compound.
Dependencies are inferred from the claim text.