Loading patent…
AssigneeNitto Denko Corporation
InventorsTakeshi Saika, Shuji Senda, Yoshitaka Kazuse, Akio Iwama, Tetsuji Sugii, Masao Nasu, Katsuji Tani, Nobuyasu Yamaguchi
Estimating the term…
A pressure sensitive adhesive sheet for the detection of microorganisms, which comprises a laminate of an adhesive layer mainly composed of a water-soluble polymer and a water-permeable membrane which does not allow passage of the microorganisms; a pressure sensitive adhesive sheet for the detection of microorganisms, wherein the surface of the adhesive layer has a contact angle with water of not more than 90°; and a method for detecting a microorganism, which comprises bringing the surface of an adhesive layer of a pressure sensitive adhesive sheet into contact with the surface of a test object, and then bringing the surface of the adhesive layer into contact with water, said adhesive layer or water containing a chromogenic reagent. The use of the laminate makes it possible to leave only stained microorganisms on the water-permeable membrane and to precisely observe the color developed by the microorganisms. The method of the present invention enables real-time monitoring without culture of microorganisms, and is not limited to the detection of viable cells alone. Inasmuch as a contact of the pressure sensitive adhesive sheet with a test face results in accumulation of microorganisms, the manipulation is simple. In addition, by properly determining a chromogenic reagent, a specific microorganism alone can be detected and counted, so that it can be applied to environmental investigation in the medical field, food industry field and the like.
A pressure sensitive adhesive sheet for the detection of microorganisms, comprising a laminate of an adhesive layer mainly composed of a water-soluble polymer and a water-permeable membrane which does not allow passage of the microorganisms, wherein said water-soluble polymer is an acrylic copolymer containing alkoxyalkyl acrylate as a monomer component and the alkoxyalkyl acrylate is contained in a proportion of 60-80 wt % of the entire acrylic copolymer and said sheet has a thickness of 10-100 μm and an adhesive strength to a Bakelite board of 30 g/12 mm width to 600 g/12 mm width.
A pressure sensitive adhesive sheet for the detection of microorganisms, comprising an adhesive layer having a contact angle with water of not more than 90° and a support, the adhesive layer being formed on the support, wherein said water-soluble polymer is an acrylic copolymer containing alkoxyalkyl acrylate as a monomer component and the alkoxyalkyl acrylate is contained in a proportion of 60-80 wt % of the entire acrylic copolymer and said sheet has a thickness of 10-100 μm and an adhesive strength to a Bakelite board of 30 g/12 mm width to 600 g/12 mm width.
Dependencies are inferred from the claim text.