Tissue factor based prothrombin time reagent
AssigneeCorvas International, Inc.
InventorScott M. Brown
Life of this patent
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Abstract
The present disclosure relates to novel liposome compositions in which tissue factor is incorporated and methods for their preparation. The composition of the phospholipids in the liposomes has been adjusted to allow maximum coagulant activity and sensitivity to extrinsic coagulation factors of the incorporated tissue factor.
Claims7
A method for incorporating active substantially purified natural or recombinant tissue factor into a lipid bilayer of phospholipid liposomes comprising the steps of:
- (a) co-solubilizing purified phospholipids, substantially purified tissue factor, and carrier proteins with detergent and cryopreservative agent and thereby causing formation of mixed micelles of phospholipids, tissue factor, carrier proteins, detergent and cryopreservative agent;
- (b) removing detergent and thereby causing the micelles to form liposomes;
- and
- (c) adding cadmium salt.
A method for incorporating substantially purified active natural or recombinant tissue into phospholipid micelles comprising the steps of:
- (a) co-solubilizing purified phospholipids, substantially purified tissue factor, and carrier proteins with detergent and thereby causing formation of mixed micelles of phospholipids, tissue factor, carrier proteins, and detergent;
- and
- (b) adding cadmium salt to said micelles, wherein the detergent is not removed from the mixed micelles.
Dependencies are inferred from the claim text.