Chelating polymers for labeling of proteins
AssigneeThe General Hospital Corporation
InventorsVladimir P. Torchilin, Vladimir S. Trubetskoy
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Abstract
A method of labelling a protein with a metal involving chelating a metal to a compound, with a molecular weight of at least 1,000, and a reactive group capable of forming a covalent bond with a target protein, and conjugating the metallically-labelled compound to a protein.
Claims36
A method for labelling a protein with a metal comprising:
- (a) providing a synthetic polypeptide to which a metal is chelated, said polypeptide having a molecular weight of at least 1,000 daltons, being purified from unchelated metal, and comprising a reactive group;
- (b) providing a protein comprising a sulfhydryl group which is either naturally occurring in said protein or the product of chemical modification;
- and
- (c) reacting said polypeptide with said protein to form a covalent bond at a single site therebetween via said reactive group and said sulfhydryl group to yield a metallically-labelled protein substantially free of unchelated metal.
A method of making a metallically-labelled complex having a molecular weight of at least 1,000 daltons, said method comprising:
- (a) providing a synthetic polypeptide, comprising a reactive group, which polypeptide covalently bonds to a protein at a single site via said reactive group, and further comprising a chelating group;
- and
- (b) contacting the polypeptide of (a) with a metal to cause chelation therewith.
A synthetic polypeptide to which a metal is chelated, having a molecular weight of at least 1,000 daltons, being purified from unchelated metal, comprising a reactive group, which polypeptide covalently bonds to a protein at a single site via said reactive group.
A metal-labelled complex, comprising a synthetic polypeptide to which a metal is chelated, having a molecular weight of at least 1,000 daltons, being purified from unchelated metal, and being covalently bonded to a protein at a single site, said protein having substantially no unchelated metal associated therewith.
Dependencies are inferred from the claim text.