Method and device for series cultivation of organisms
AssigneesForschungszentrum Julich GmbH, Dasgip Drescher Arnold, Schneider Gesellschaft fur Informations-und Prozesstechnologie mbH
InventorsThomas Drescher, Juta Altenbach-Rehm, Dirk Weuster-Botz
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Abstract
The invention relates to a method and a device for series cultivation of organisms which is particularly suitable for providing organisms to be cultivated with a culture medium that has a toxic effect on said organisms at a high dosage, but when applied in appropriate dosage, said device determines the amount of substrate required to enable organisms to produce substances microbially in optimum conditions. According to the invention, a dosing schedule is predefined and the amount of substrate in the flask is adjusted according to actual requirements. In order to avoid underdosage, filling of the shaking flask no longer occurs according to a fixed predefined sequence.
Claims26
Process for dosing liquids in which a liquid from a liquid supply tank (8) is dosed into at least one container by means of a dosing device (5) and in which the amount of liquid to be filled into the container is stored in a computer (7), characterized by the following steps:
- programming a dosing schedule for a container,
- filling a container with a volume prescribed according to a dosing schedule
- calculating and storing the volume of liquid added
- adding another volume of liquid that is calculated on the basis of the amount already dosed and stored in the computer as well as on the basis of the dosing schedule, and thus that at the point in time of this dosing, corresponds to the actual requirement at a dosing point in time at which the calculated amount of liquid actually to be dosed does not fall below a minimum dosing volume that is the least that can be dosed.
Device comprising a liquid supply tank (8), a dosing device (5), a computer (7), a feed line (4) connected to the dosing device (5) and to the liquid supply tank (8) as well as at least one container that is connected to the dosing device (5) via a supply line (2), characterized in that
- the computer (7) is connected to the dosing device (5) as well as to valves that open and close the supply lines (2) via control lines, whereby a dosing schedule programmed for each container is programmed in the computer (7), so as to control the filling of a container as follows:
- filling a container with a volume prescribed according to a dosing schedule
- calculating and storing the volume of liquid added
- adding another volume of liquid that is calculated on the basis of the amount already dosed and stored in the computer as well as on the basis of the dosing schedule, and thus that at the point in time of this dosing, it corresponds to the actual requirement at a dosing point in time at which the calculated amount of liquid actually to be dosed does not fall below a minimum dosing volume that is the least that can be dosed.
A computer-controlled process for feeding a plurality of liquid doses to a plurality of biological media, wherein adequate flow properties for dispensing the liquid doses essentially in their entirety to each biological medium cannot be obtained unless each liquid dose is at least as great as a predetermined minimum volume, said process comprising:
- a. selecting an effective feed rate for at least one of the biological media and selecting a minimum volume for each liquid dose, and storing at least one feed rate and said minimum volume in the computer as minimum dose volume data and as data representing an amount dispensed-vs.-time curve,
- b. in response to a signal from the computer based on stored data related to a feed rate, delivering in its entirety a first liquid dose, at least as large in volume as said predetermined minimum volume, to a first biological medium, in accordance with said feed rate,
- c. generating computer data corresponding to a measurement of the volume of liquid delivered to the first biological medium in said step (b) and storing the thus-generated data in said computer,
- d. repeating said steps (b) and (c) with respect to the other biological media, thereby generating body of stored data in said computer corresponding to the measurements of volume of liquid dispensed,
- e. generating with said computer calculated dose-timing data, based upon said thus-generated data of steps (c) and the predetermined feed rate related to said first biological medium, and controlling the timing of the dispensing of a second liquid dose to said first biological medium so as to conform essentially to said amount-dispensed vs. time curve for that biological medium and so as to conform to said minimum dose volume data,
- f. repeating said step (e) for the other biological media and with respect to the relevant feed rate for each biological medium.
Process for dosing liquids in which a liquid is dosed into at least one container and in which the amount of liquid to be dosed into the container is stored in a computer, characterized by the following steps:
- programming a dosing schedule for a container,
- filling the container with a volume prescribed according to the dosing schedule
- calculating and storing the volume of liquid added
- adding another volume of liquid that is calculated on the basis of the amount already dosed and stored in the computer, as well as on the basis of the dosing schedule, such that the dosing corresponds to the actual requirement at that point in time, but wherein the actual dosing time does not precede the time calculated to be the time at which at least a minimum dosing volume is required, wherein the minimum dosing volume is the least that can be dosed.
Device comprising a dosing device, a computer, at least one tubing connecting the dosing device to at least one container, and one valve for each of the at least one tubings directly connected to each of the at least one container and the computer is connected to the dosing device and to all of the at least one valves, whereby a dosing schedule programmed for each container is programmed in the computer, so as to control the filing of the at least one container as follows:
- filing the container with a volume according to a dosing schedule
- calculating and storing the volume of liquid added
- adding another volume of liquid that is calculated on the basis of the amount already dosed and stored in the computer, as well as on the basis of the dosing schedule, such that the dosing corresponds to the actual requirement at that point in time, but wherein the actual dosing time does not precede the time calculated to be the time at which at least a minimum dosing volume is required, wherein the minimum dosing volume is the least that can be dosed.
Dependencies are inferred from the claim text.