Genetic induction of anti-viral immune response and genetic vaccine for filovirus
AssigneePowerJect Vaccines Inc.
InventorsJoel R. Haynes, Connie S. Schmaljohn, Deborah L. Fuller, Alan Schmaljohn, Peter B. Jahrling
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Abstract
An approach to genetic vaccine methodology is described. A genetic construction encoding antigenic determinants of a filovirus is transfected into cells of the vaccinated individuals using a particle acceleration protocol so as to express the viral antigens in healthy cells to produce an immune response to those antigens.
Claims11
A method of inducing an immune response to a Marburg or Ebola virus glycoprotein in a mammal, said method comprising:
- (a) providing a genetic construction comprising a promoter operative in cells of the mammal and a coding region for a determinant of the glycoprotein, the genetic construction not comprising sequences necessary for replication of the virus;
- (b) coating copies of the genetic construction onto carrier particles small in size in relation to the size of the cells of the mammal;
- and
- (c) accelerating the coated carrier particles into epidermal cells of the mammal in vivo, thereby inducing an immune response against the glycoprotein.
A composition of matter comprising a carrier particle and a genetic construction coated onto the carrier particle, wherein the genetic construction comprises a promoter operative in the cells of a mammal and a coding region for a determinant of a Marburg or Ebola virus glycoprotein.
Dependencies are inferred from the claim text.