Swine vesicular disease virus and mutant strains and preparation process and use thereof
AssigneeDevelopment Center for Biotechnology
InventorsChing Long Hwong, Cheng-Kai Lo, Ying-Chuan Yang, King-Song Jeng, Edward L. Chang
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Abstract
The present invention relates to a gene of swine vesicular disease virus (SVDV) and the mutant strains of the gene, and the expression plasmids, the preparation process thereof. The invention also relates to a vaccine for use in the prophylaxis of swine vesicular disease composition containing the mutant strains. Furthermore, the invention provides a process for differentiating mutant strains of SVDV from the wild type strain of SVDV, coxsackievirus and foot-and-mouth disease virus by polymerase chain reaction.
Claims28
An isolated DNA molecule encoding the full genome of the Taiwan Yu-Li strain of SVDV, which comprises the sequence of SEQ ID NO:
- 1, as follows:
- (SEQ ID NO:1) 1 TTAAAACAGC CTGTGGGTTG TTCCCACCCA CAGGGCCCAC TGGGCGCTAG 51 CACACTGGTA TCACGGTACC TTTGTGCGCC TGTTTGACTT ACCCTCCCCA 101 AACGCAACTT AGAAGCACAA CTTAAATGGT CAATAGACGG CTCAGTATGC 151 CAACTGAGTC TCGATCAAGC ACTTCTGTTA CCCCGGACTG AGTACCAATA 201 GGCTGCTCAC CCGGCTGAAG GGGAAACCGT TCGTTACCCG ACTAACTACT 251 TCGAGAAACC TAGTACCACC ATGAAAGTTG CGCACGTTTC GTTCCGCACA 301 ACCCCAGTGT AGATCAGGCC GATGAGTCAC CCCAAACCCC ACGGGCGACC 351 GTGGCGGTGG CTGCGCTGGC GGCCTGCCCA TGGGGCAACT CATGGGATGC 401 TTCAATACTG ACATGGTGCG AAGAGTCTAT TGAGCTAGTT GGTAGTCCTC 451 CGGCCCCTGA ATGCGGCTAA TCCTAACTGC GGAGCAGATA CCCACGCACC 501 AGTGGGCAGT CTGTCGTAAT GGGCAACTCT GCAGCGGAAC CGACTACTTT 551 GGGTGTCCGT GTTTCCTTTT GTTCTTATAC TGGCTACTTA TGGTGACAAT 601 TGAGAGATTG TAACCATATT GCTATTGGAT TGGCCACCTG GCGACGAATA 651 GAACAGTTGC TTACCTGTTT GTTGGTCTCG TATCACTGAA CTACAAAGCC 701 TTAAACACCC TTTAATTTCA TCATAACGCT CAATACGTTA AAATGGGAGC 751 TCAAGTGTCA ACACAAAAGA CCGGTGCTCA TGAGACCAGC TTGAGTGCAG 801 CGGGCAACTC AGTCATTCAT TACACAAACA TAAACTACTA CAAGGATGCT 851 GCTTCAAATT CAGCAAATAG ACAAGACTTC ACACAGGACC CGGGGAAGTT 901 CACCGAACCT GTGAAAGACA TCATGGTCAA ATCATCGCCT GCTCTCAATT 951 CCCCATCAGC AGAGGAGTGT GGCTACAGTG ACAGGGTAAG ATCCATCACC 1001 TTAGGGAATT CGACCATAAC AACTCAAGAA TGTGCAAACG TGGTAGTTGG 1051 ATATGGGGTG TGGCCAACTT ACTTGAAGGA TGAAGAGGCA ACAGCAGAGG 1101 ATCAACCCAC TCAACCAGAT GTGGCCACGT GCAGGTTTTA CACGCTCGAA 1151 TCCGTGATGT GGCAACAGAG TTCACCAGGC TGGTGGTGGA AGTTCCCTGA 1201 CGCGTTGTCC AACATGGGGC TATTTGGGCA AAATATGCAG TACCACTACC 1251 TTGGGAGAGC CGGATACACG ATACACGTGC AGTGCAACGC GTCCAAATTT 1301 CACCAAGGGT GTCTGCTGGT GGTATGTGTG CCAGAAGCAG AGATGGGGTG 1351 TGCCACGTTG GCCAATAAGC CTGACCCAAA AAGCCTGAGT AAAGGGGAAA 1401 TAGCCAACAT GTTTGAATCC CAAAGCTCCA CCGGGGAAAC GGCCGTGCAA 1451 GCTAATGTGA TCAATGCAGG CATGGGTGTT GGTGTTGGTA ATCTAACTAT 1501 CTTCCCCCAC CAGTGGATCA ACTTGCGCAC TAACAAVAGC GCTACGATTG 1551 TCATGCCATA TATAAACAGC GTGCCCATGG ACAACATGTT CAGACACAAC 1601 AATTTTACAC TCATGGTCAT CCCGTTCGCC CCACTGAGCT ACAGCACAGG 1651 GGCTACCACG TACGTACCAA TCACTGTGAC AGTGGCGCCA ATGTGCGCTG 1701 AATATAATGG GCTGCGTCTG GCCGGCAAGC AAGGTTTACC AACGCTGTCG 1751 ACACCCGGGA GCAACCAGTT TCTCACGTCC GATGACTTCC AGTCACCATC 1801 AGCCATGCCA CAATTCGATG TCACTCCTGA GATGGATATT CCAGGACAAG 1851 TCAACAACTT GATGGAGATT GCAGAAGTAG ATTCTGTAGT GCCTGTAAAC 1901 AACACAGAAG GGAAAGTGAT GTCAATTGAG GCATACCAGA TACCTGTGCA 1951 ATCGAATCCA ACCAACGGTT CTCAGGTTTT TGGGTTCCCA TTGACCCCAG 2001 GGGCCAATAG TGTGTTAAAC AGGACTTTGC TGGGAGAAAT CTTAAACTAC 2051 TATGCCCATT GGTCAGGCAG CATCAAACTA ACATTTATGT TTTGCGGGTC 2101 AGCGATGGCT ACAGGAAAAT TCTTACTGGC ATACTCACCA CCGGGAGCTG 2151 GGGCACCGAC CACACGCAAG GAGGCGATGC TAGGTACTCA CGTGATCTGG 2201 GATGTGGGTC TACAATCGAG CTGCGTATTG TGTATACCAT GGATTAGTCA 2251 AACGCACTAC AGGTATGTAG TAATGGATGA ATACACCGCT GGTGGATACA 2301 TAACTTGCTG GTATCAAACA AATATTGTGG TGCCTGCAGA TGCACAGAGT 2351 GACTGTAAGA TCTTGTGTTT TGTGTCGGCA TGTAACGATT TCTCAGTTAG 2401 GATGCTCAAG GACACACCCT TTATAAAACA GGATAATTTC TTCCAAGGGC 2451 CCCCAGGAGA GGTGATGGAA AGAGCCGTTG CCCGCGTCGC TGATACCATT 2501 GGGAGCGGAC CAGTTAACTC GGAATCCATT CCAGCTCTAA CCGCCGCAGA 2551 GACAGGGCAC ACGTCACAAG TTGTACCATC AGACACAATG CAAACTAGGC 2601 ACGTGAAGAA TTATCATTCA AGGTCAGAGT CGACAGTGGA GAACTTCCTG 2651 TGCAGATCTG CATGCGTCTT CTACACCACA TACAAGAACC ATGGCTCTGA 2701 TGGCGACAAC TTCGCCTACT GGGTAATCAA CACACGGCAA GTTGCTCAAC 2751 TGCGTCGGAA GCTCGAAATG TTCACGTACG CAAGATTTGA TCTGGAGTTG 2801 ACCTTCGTGA TCACTAGCAC TCAGGAACAA CCCACCGTTA AAGGTCAAGA 2851 TACACCAGTG CTCACCCACC AAATAATGTA TGTACCTCCA GGTGGTCCAG 2901 TACCCACAAA GGTAAACAGC TACAGCTGGC AAACGTCCAC CAACCCAAGT 2951 GTGTTCTGGA CGGAAGGGAG CGCACCGCCT CGAATGTCGA TACTATTCAT 3001 TGGCATAGGC AACGCATACA GCATGTTCTA TGACGGGTGG GCCAGGTTTG 3051 ACAAGCAAGG GACATACGGC GTCCAAGCAC TAAACAACAT GGGGACACTA 3101 TATATGAGAC ATGTGAATGA TGGGGGTCCC GGTCCCATTG TGAGCACAGT 3151 ACGAATTTAC TTCAAGCCAA AGCACGTCAA AACGTGGGTC CCAAGACCGC 3201 CCAGACTATG TCAATACCAA AAGGCTGGCA ACGTGAATTT TGAACCCACT 3251 GGTGTGACTG AGGGTAGGAC AGATATAACA ACCATGAAAA CCACTGGCGC 3301 CTTCGGGCAG CAGTCTGGTG CCGTGTACGT TGGCAACTAT AGAGTGGTGA 3351 ATAGACATCT CGCAACGCGC GCGGACTGGC AAAACTGTGT GTGGGAAGAC 3401 TACAACAGAG ACCTTCTAGT GAGCACCACC ACTGCACATG GCTGCGACAC 3451 CATTGCCAGG TGCGATTGCA CAGCAGGAGT GTACTTCTGC GCCTCCAGAA 3501 ACAAGCACTA TCCAGTCACA TTTGAGGGGC CCGGTCTTGT GAAGGTTCAA 3551 GAGAGTGAGT ATTACCCGAA AAAGTACCAA TCCCATGTAC TGCTCGCAGC 3601 TGGATTTGCA GAGCCGGGTG ATTGTGGAGG GATTCTCAGA TGCCAACATG 3561 GGGTGATTGG CATAGTTACC GTGGGGGGGG AAGGTGTTGT TGGTTTTGCC 3701 GATGTAAGAG ACTTGTTGTG GCTGGAGGAC GATGCCATGG AGCAAGGAGT 3751 TAGGGATTAT GTGGAACAAC TCGGCAACTG CTTCGGCTCA GGATTCACCA 3801 ATCAAATTTG CGAACAGGTT ACCCTTCTAA AAGAGTCGTT AATTGGACAG 3851 GATTCTATCC TTGAGAAGTC TCTCAAGGCC CTCGTCAAGA TAGTATCAGC 3901 ACTCGTGATC GTGGTGAGAA ATCACGATGA CCTCATTACG GTCACCGCCA 3951 CACTGGCGTT AATAGGATGT ACCACCTCAC CATGGCGCTG GCTCAAGCAG 4001 AAAGTGTCTC AGTACTATGG CATCCCCATG GCTGAAAGGC AAAATAGTGG 4051 CTGGTTAAAG AAGTTCACAG AGATGACCAA TGCCTGTAAG GGCATGGAGT 4101 GGATAGCCAT CAAGATCCAA AAATTCATAG AGTGGTTGAA GGTTAAGATC 4151 CTGCCAGAAG TCAAGGAAAA GCATGAGTTC CTCAACAGGC TTAAACAACT 4201 ACCACTCTTG GAAAGTCAAA TAGCAACTAT TGAGCAGAGT GCACCATCTC 4251 AAAGTGACCA GGAGCAACTA TTCTCTAATG TACAGTACTT TGCCCACTAC 4301 TGTCGGAAGT ATGCACCATT GTACGCCGCT GAAGCAAAGA GAGTGTTCTC 4351 ACTTGAAAAG AAGATGAGCA ATTACATACA GTTCAAGTCC AAATGCCGTA 4401 TTGAACCCGT CTGTCTCTTG CTCCATGGCA GCCCAGGCGC TGGGAAGTCT 4451 GTGGCAACGA ACTTGATTGG GCGCTCGCTC GCTGAGAAAC TCAACAGCTC 4501 GGTGTACTCA CTACCACCAG ATCCAGACCA TTTCGATGGT TACAAACAGC 4551 AAGCTGTTGT CATCATGGAC GACTTGTGCC AGAACCCGGA CGGTAAAGAT 4601 GTGTCCTTGT TCTGTCAGAT GGTCTCCAGC GTTGACTTCG TGCCTCCCAT 4651 GGCGGCGCTT GAGGAAAAAG GCATTCTATT CACCTCGCCG TTCGTTCTCG 4701 CGTCCACCAA TGCAGGGTCA GTTAACGCCC CCACGGTCTC CGACAGTAGA 4751 GCACTCGTAA GAAGGTTCCA TTTTGACATG AACATCGAGG TTATTTCCAT 4801 GTATAGCCAG AACGGTAAGA TCAACATGCC TATGGCAGTT AAAACATGTG 4851 ATGAGGAGTG TTGCCCGGTC AACTTCAAAA AGTGCTGCCC ACTAGTGTGT 4901 GGCAAAGCTA TACAATTCAT AGACAGGAGG ACCCAAGTTA GGTATTCATT 4951 GGACATGCTG GTTACCGAAA TGTTTAGGGA GTACAATCAC ACACACAGTC 5001 TGGGGGCCAC CCTCGAGGCA TTGTTCCAAG GACCACCAGT TTATAGAGAG 5051 ATCAAAATCA GTGTTGCCCC AGAAACTCCT CCACCACCAG CAGTTGCCGA 5101 CTTACTAAAA TCAGTAGACA GTGAGGCTGT GAGGGAGTAC TGCAAGGAGA 5151 AAGGGTGGCT TATACCAGAG GTCGATTCCA CCCTACAGAT AGAAAAGCAT 5201 GTGAGCAGAG CGTTCATATG TTTGCAAGCT CTAACCACAT TTGTCTCGGT 5251 TGCAGGCATA ATATACATCA TCTACAAATT GTTTGCAGGT TTCCAAGGCG 5301 CATACACAGG GATGCCTAAT CAGAAGCCCA AGGTGCCCAC CCTGAGACAA 5351 GCCAAAGTGC AGGGTCCAGC GTTTGAGTTC GCCGTGGCGA TGATGAAAAG 5401 AAACGCCAGT ACAGTGAAAA CTGAGTATGG TGAATTCACC ATGCTTGGGA 5451 TTTACGACAG GTGGGCGGTG TTGCCACGCC ATGCCAAACC TGGCCCCACC 5501 ATCTTGATGA ACGACCAGGT AGTCGGAGTG TTGGACGCCA AGGAACTAGT 5551 TGATAAAGAT GGGACCAACC TGGAATTGAC TCTCTTGAAG CTCAACCGCA 5601 ACGAGAAGTT TAGAGACATC AGGGGATTCT TAGCACGAGA GGAGGTCGAA 5651 GTGAACGAAG CTGTCCTAGC AATAAACACA AGTAAATTCC CGAATATGTA 5701 CATACCCGTG GGCCGGGTAA CCGACTATGG GTTCTTAAAT CTGGGTGGAA 5751 CCCCCACGAA GAGAATGCTC ATGTACAATT TCCCAACTAG GGCAGGCCAG 5801 TGTGGGGGTG TCCTTATGTC AACAGGGAAA GTCCTGGGAA TACATGTAGG 5851 AGGGAATGGA CACCAAGGGT TTTCAGCGGC ACTCCTCAGA CACTACTTCA 5901 ATGAGGAGCA GGGTGAGATA GAATTCATTG AGAGCTCAAA GGACGCAGGA 5951 TTTCCCGTGA TCAACACTCC CAGCAAGACA AAATTGGAAC CAAGTGTGTT 6001 TCACCACGTG TTCGAGGGCA ACAAGGAACC AGCGGTTCTC AGAAATGGGG 6051 ACCCACGACT CAAGGCCAAC TTTGAGGAGG CAATCTTCTC CAAGTACATT 6101 GGCAATGTTA ACACACATGT AGACGAGTAC ATGATGGAGG CTGTAGATCA 6151 TTATGCAGGA CAACTAGCCA CACTGGACAT CAGCACGGAA CCCATGAAGC 6201 TAGAAGATGC CGTGTATGGC ACTGAGGGGC TCGAAGCACT AGACCTGACC 6251 ACCAGTGCAG GTTACCCTTA TGTGGCCCTG GGTATCAAGA AAAGAGACAT 6301 CCTATCCAAG AAGACCAGAG ACCTTACCAA GCTAAAGGAA TGCATGGACA 6351 AATATGGTCT AAACTTGCCA ATGGTAACCT ATGTCAAGGA CGAGTTGAGA 6401 TCTGCCGACA AAGTGGCCAA GGGAAAATCC AGGCTCATCG AGGCTTCTAG 6451 CCTCAACGAC TCAGTAGCAA TGAGGCAGAC ATTTGGAAAC CTATATAAGA 6501 CTTTCCACCT CAACCCGGGC ATCGTTACGG GTAGCGCCGT TGGGTGTGAC 6551 CCAGATGTCT TTTGGAGCAA GATCCCCGTT ATGCTCGATG GACATGTCAT 6601 AGCGTTTGAC TATTCAGGCT ATGACGCCAG CCTCAGCCCA GTGTGGTTTA 6651 CGTGCTTGAA ACTCCTCCTG GAGAAGCTAG GGTACACAAA CAAGGAAACG 6701 AACTACATAG ACTACCTCTG TAATTCCCAC CACCTGTACA GGGACAAACA 6751 CTACTTTGTG AGGGGCGGCA TGCCATCAGG ATGCTCAGGC ACTAGCATAT 6801 TTAATTCCAT GATTAACAAC ATCATAATCA GAACCCTCAT GCTGAAGGTT 6851 TATAAAGGCA TTGATTTGGA CCAATTCAGA ATGATTGCAT ATGGGGATGA 6901 TGTGATAGCT TCATACCCGT GGCCCATCGA TGCCTCACTG CTAGCTGAAG 6951 CAGGGAAGGA TTGTGGCTTG ATCATGACCC CAGCAGATAA AGGCGAGTGT 7001 TTCAATGAGG TAACCTGGAC AAACATGACC TTCCTGAAAA GGTACTTCAG 7051 GGCAGATGAA CAGTACCCAT TTTTGGTCCA TCCTGTCATG CCAATGAAGG 7101 ATATACACGA ATCCATTAGG TGGACTAAAG ATCCTAAGAA CACACAGGAT 7151 CACGTGCGCT CGCTGTGTTT ATTGGCTTGG CACAACGGGG AGCACGAATA 7201 TGAGGAGTTT ATTCGTAAGA TCAGAAGCGT GCCCGTAGGG CGCTGCTTGT 7251 CCCTCCCTGC GTTTTCAACG CTGCGCAGGA AGTGGTTGGA CTCCTTTTAA 7301 AATTAGAGCA CAATTAGTCA ATCATAATTG GCTCAACCCT ACCGCATGAA 7351 CCGAACTTGA TAAAAGTGCG GTAGGGGTAA ATTCTCCGTA TTCGGTGCGG;
- or a degenerate sequence which encodes the same amino acid sequence as SEQ ID NO:
- 1.
An isolated DNA molecule encoding a mutant strain of SVDV, comprising a sequence of SEQ ID NO:
- 1 with the bases at positions 2705-2710 replaced with GAAAGC (SEQ ID NO:
- 5), or a degenerate sequence which encodes the same amino acid sequence.
An isolated DNA molecule encoding a mutant strain of SVDV, comprising a sequence of SEQ ID NO:
- 1 with the bases at positions 2693-2710 replaced with GACAACGGCGCTGAAAGC (SEQ ID NO:
- 6), or a degenerate sequence which encodes the same amino acid sequence.
An isolated DNA molecule encoding a mutant strain of SVDV, comprising a sequence of SEQ ID NO:
- 1 with the bases at positions 2693-2710 replaced with GGCTCCACCACAAACAAGGATAAGAGC (SEQ ID NO:
- 7), or a degenerate sequence which encodes the same amino acid sequence.
An isolated DNA molecule encoding a mutant strain of SVDV, comprising a sequence of SEQ ID NO:
- 1 with a substituted base at one or more of positions 2693-2710.
A mutant strain of SVDV, comprising a genome encoded by a DNA molecule comprising a sequence of SEQ, ID NO:
- 1 with a substituted base at one or more of positions 2693-2710.
A mutant strain of SVDV, comprising a genome encoded by a DNA molecule comprising a sequence of SEQ ID NO:
- 1 with the bases at positions 2705-2710 replaced with GAAAGC (SEQ ID NO:
- 5), or a degenerate sequence which encodes the same amino acid sequence.
A mutant strain of SVDV, comprising a genome encoded by a DNA molecule comprising a sequence of SEQ ID NO:
- 1 with the bases at positions 2693-2710 replaced with GACAACGGCGCTGAAAGC (SEQ ID NO:
- 6), or a degenerate sequence which encodes the same amino acid sequence.
A mutant strain of SVDV, comprising a genome encoded by a DNA molecule comprising a sequence of SEQ ID NO:
- 1 with the bases at positions 2693-2710 replaced with GGCTCCACCACAAACAAGGATAAGAGC (SEQ ID NO:
- 7), or a degenerate sequence which encodes the same amino acid sequence.
An expression plasmid comprising a sequence of SEQ ID NO:
- 1, or a degenerate sequence which encodes the same amino acid sequence as SEQ ID NO:
- 1.
An expression plasmid comprising a sequence of SEQ ID NO:
- 1 with a substituted base at one or more of positions 2693-2710.
An expression plasmid comprising a sequence of SEQ ID NO:
- 1 that has been mutagenized at one or more bases encoding an amino acid at position 83, 84, 85, 86, 87, or 88 of the capsid protein VP1 (nucleotide position 2693-2710).
An expression plasmid comprising pCI/SVDV-T/SP7 deposited on Aug. 4, 1998, with China Center for Type Culture Collection, Luo Jia Shan, Wuhan, the People's Republic of China, under Accession No. CCTCC M 98012.
An expression plasmid comprising pCI/SVDV-T/H21 deposited on Aug. 4, 1998, with China Center for Type Culture Collection, Luo Jia Shan, Wuhan, The People's Republic of China, under Accession No. CCTCC M 98011.
An expression plasmid comprising pCI/SVDV-T/N3 deposited on Aug. 4, 1998, with China Center for Type Culture Collection, Luo Jia Shan, Wuhan, the People's Republic of China, under Accession No. CCTCC M 98010.
An immunogenic composition for use in prophylaxis of the SVDV, comprising the Taiwan Yu-Li strain of SVDV and an adjuvant for the composition.
An immunogenic composition for use in prophylaxis of the SVDV, comprising an adjuvant and a mutant strain of SVDV, the mutant strain comprising a genome encoded by a DNA molecule comprising a sequence of SEQ ID NO:
- 1 with a substituted base at one or more of positions 2693-2710.
An immunogenic composition for use in prophylaxis of the SVDV, comprising an adjuvant and a mutant strain of SVDV, the mutant strain comprising a genome encoded by a DNA molecule comprising a sequence of SEQ ID NO:
- 1 with the bases at positions 2705-2710 replaced with GAAAGC (SEQ ID NO:
- 5), or a degenerate sequence which encodes the same amino acid sequence.
An immunogenic composition for use in prophylaxis of the SVDV, comprising an adjuvant and a mutant strain of SVDV, the mutant strain comprising a genome encoded by a DNA molecule comprising a sequence of SEQ ID NO:
- 1 with the bases at positions 2693-2710 replaced with GACAACGGCGCTGAAAGC (SEQ ID NO:
- 6), or a degenerate sequence which encodes the same amino acid sequence.
An immunogenic composition for use in prophylaxis of the SVDV, comprising an adjuvant and a mutant strain of SVDV, the mutant strain comprising a genome encoded by a DNA molecule comprising a sequence of SEQ ID NO:
- 1 with the bases at positions 2693-2710 replaced with GGCTCCACCACAAACAAGGATAAGAGC (SEQ ID NO:
- 7), or a degenerate sequence which encodes the same amino acid sequence.
A mutant strain of SVDV, wherein the mutant strain has been altered so that two or more amino acids in VP1 positions 83-88 are different from a wild-type strain.
A mutant strain of SVDV, wherein the mutant strain has been altered so that two or more codons in the region corresponding to nucleotides 2693-2710 of SEQ ID NO 1 encode amino acids different from a wild-type strain.
Dependencies are inferred from the claim text.