GA 20-oxidase gene sequences
AssigneeLong Ashton Research Station
InventorsTheodor Lange, Jan E. Graebe, Peter Hedden, Andrew Phillips
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Abstract
The invention relates to the molecular cloning and expression of a gibberellin (GA) 20-oxidase gene and its use, for example in transgenic plants. Aspects of the invention include recombinant DNA which encodes a polypeptide exhibiting GA 20-oxidase activity, a recombinant polypeptide exhibiting GA 20-oxidase activity, and transgenic plants which express a GA 20-oxidase gene or reverse GA 20-oxidase sequences.
Claims19
A transgenic plant or seed comprising stably integrated into the plant's or seed's genome, (a) a DNA sequence which encodes a polypcptide exhibiting GA 20-oxidase activity having the amino acid sequence according to SEQ ID NO. 2, 4 or 6, or (b) a DNA sequence which hybridizes with the DNA sequence of (a) or its complement under hybridizing conditions and which encodes a polypeptide exhibiting GA 20-oxidase activity.
A transgenic plant or seed comprising, stably integrated into the plant's or seed's genome, a chimaeric gene construct comprising at least a part of (a) a DNA sequence which encodes a polypeptide exhibiting GA 20-oxidase activity having the amino acid sequence according to SEQ ID No. 2, 4, or 6, or (b) a DNA sequence which hybrizes with the DNA sequence of (a) or its complement under hybridizino conditions and which encodes a polypeptide exhibiting GA 20-oxidase activity, said DNA sequence (a) or (b) being operably linked in reverse orientation with plant expression signals comprising promoter and termination sequences causing the reverse DNA sequence of (a) or (b) to express antisense mRNA within a plant and said part being of a length surncient to reduce the expression of a DNA encodina a polypeptide exhibiting GA 20-oxidase activity.
Dependencies are inferred from the claim text.