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AssigneesThe Trustees of the University of Pennsylvania, Apollan, Inc.
InventorsRichard A. Carrano, Bin Wang, David B. Weiner
Estimating the term…
Methods of introducing genetic material into cells of an individual and compositions and kits for practicing the same are disclosed. The methods comprise the steps of contacting cells of an individual with a genetic vaccine facilitator and administering to the cells, a nucleic acid molecule that is free of retroviral particles. The nucleic acid molecule comprises a nucleotide sequence that encodes a protein that comprises at least one epitope that is identical or substantially similar to an epitope of a pathogen antigen or an antigen associated with a hyperproliferative or autoimmune disease, a protein otherwise missing from the individual due to a missing, non-functional or partially functioning gene, or a protein that produce a therapeutic effect on an individual. Methods of prophylactically and therapeutically immunizing an individual against HIV are disclosed. Pharmaceutical compositions and kits for practicing methods of the present invention are disclosed.
A method of introducing nucleic acid molecules into cells of an individual comprising the step of administering in vivo to cells in said individual's body urea and nucleic acid molecules wherein said nucleic acid molecules are taken up by said cells.
A method of generating an immune response in an individual against an antigen comprising administering in vivo to cells of said individual's body urea and DNA molecules that comprise a DNA sequence that encodes said antigen, said DNA sequence operatively linked to regulatory sequences which control the expression of said DNA sequence;
A pharmaceutical composition comprising urea and DNA molecules that comprise a nuclcotide sequence which encodes a protein wherein said nucleotide sequence is operably linked to regulatory sequences required for expression in a mammal.
A pharmaceutical kit comprising:
A method of delivering a protein into cells of an individual in vivo comprising administering to cells of said individual's body, urea and DNA molecules that comprise a DNA sequence that encodes said protein, said DNA sequence operatively linked to regulatory sequences which control the expression of said DNA sequence;
Dependencies are inferred from the claim text.