Enzymes with xylanase activity from Aspergillus aculeatus
AssigneeNovo Nordisk A/S
InventorsLene Venke Kofod, MArkus Sakari Kauppinen, Stephan Christgau, Hans Peter Heldt-Hansen, Henrik Dalbøge, Lene Nonboe Andersen, Joan Qi Si, Tina Sejersgård Jacobsen, Niels Munk, Anette Müllertz
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Abstract
A DNA construct encoding an enzyme(s) exhibiting xylanase activity, which enzyme is immunologically reactive with antibody raised against a purified xylanase derived from Aspergillus aculealus, CBS 101.43, recombinant vectors and cells comprising said construct and a method for producing said enzyme using said cell comprising said construct.
Claims16
A DNA construct which comprises a DNA sequence encoding an enzyme having xylanase activity, as measured by release of reducing sugars from birch xylan or by release of blue color from AZCL-Birch xylan, said enzyme having a pH optimum of 4-5 and a temperature optimum of 70-80° C. and which DNA sequence hybridizes to a DNA sequence depicted in SEQ ID NO. 3 under the following conditions:
- hybridizing in 5×SSC, 5×Denhardt's solution, 50 mM sodium phosphate, pH 6.8 and 50 ug denatured sonicated calf thymus DNA for 18 hrs at about 40° C. followed by washing three times in 2×SSC, 0.2% SDS at 40° C. for 30 minutes.
A DNA construct which comprises a DNA sequence encoding an enzyme having xylanase activity and an amino acid sequence depicted in SEQ ID NO. 4.
Dependencies are inferred from the claim text.