Method of imaging cell death in vivo
AssigneesThe Board of Trustees of the Leland Stanford Junior University, University of Washington
InventorsFrancis G. Blankenberg, H. William Strauss, Jonathan F. Tait, Peter D. Katsikis
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Abstract
A method of imaging apoptosis in vivo, using radiolabeled annexin, is described.
Claims75
A method of imaging cell death in a nucleated cell within a region of a mammalian subject in vivo, comprising
- (a) administering to the subject, annexin labeled with a biocompatible radionuclide,
- (b) positioning the subject within the detection field of a radiation detector device, and
- (c) measuring radiation emission from the radionuclide in the subject, with the radiation detector device, to construct an image of radiation emission,
- wherein said image is a representation of cell death in said nucleated cell of said mammalian subject.
A method of imaging cell death in a nucleated cell within a region of a mammalian subject in vivo, comprising
- (a) administering to the subject, annexin labeled with a biocompatible radionuclide,
- (b) measuring radiation emission from the radionuclide in the subject, to construct an image of radiation emission,
- wherein said image is a representation of cell death in said nucleated cell of said mammalian subject.
A method of imaging cell death in a tumor in a mammalian subject in vivo, comprising
- (a) administering to the subject, annexin labeled with a biocompatible radionuclide,
- (b) measuring radiation emission from the radionuclide in the subject, to construct an image of radiation emission,
- wherein said image is a representation of cell death in said tumor in said mammalian subject.
A method of imaging cell death in a nucleated cell within a region of a mammalian subject in vivo, comprising
- (a) administering to the subject, a dose of annexin labeled with a biocompatible radionuclide, said dose being less than 300 μg protein/kg
- (b) measuring radiation emission from the radionuclide in the subject, to construct an image of radiation emission, said measuring being done between about 5 minutes and about 1 day after administration of the annexin,
- wherein said image is a representation of cell death in said nucleated cell of said mammalian subject.
A method of imaging cell death in a transplant in a mammalian subject in vivo, comprising
- (a) administering to the subject, annexin labeled with a biocompatible radionuclide,
- (b) measuring radiation emission from the radionuclide in the subject, to construct an image of radiation emission,
- wherein said image is a representation of cell death in said transplant in said mammalian subject.
A method of imaging cell death in an ischemic site in a mammalian subject in vivo, comprising
- (a) administering to the subject, annexin labeled with a biocompatible radionuclide,
- (b) measuring radiation emission from the radionuclide in the subject, to construct an image of radiation emission,
- wherein said image is a representation of cell death in said ischemic site in said mammalian subject.
Dependencies are inferred from the claim text.