Integrated miniaturized device for processing and NMR detection of liquid phase samples

A miniaturized total analysis system for liquid phase sample preparation and detection, comprising:

• a microfabricated support body having first and second component halves each having substantially planar opposing interior and exterior surfaces;
• a first microchannel microfabricated in the interior surface of the first support body half and a second microchannel microfabricated in the interior surface of the second support body half, wherein each of the microchannels is so arranged as to provide the mirror image of the other;
• an elongate bore formed by aligning the interior surfaces of the support body halves in facing abutment with each other whereby the microchannels define the elongate bore;
• an inlet port and an outlet port communicating with the elongate bore, the ports enabling the downstream passage of fluid from an external source through the elongate bore;
• and
• downstream from the elongate bore and in fluid communication therewith, an NMR detection compartment around which is an NMR rf microcoil, wherein the NMR detection compartment and the NMR rf microcoil are housed within the support body.

A miniaturized total analysis system for liquid phase sample preparation and detection, comprising:

• a microfabricated support body having first and second component halves each having substantially planar opposing interior and exterior surfaces;
• a first microchannel microfabricated in the interior surface of the first support body half and a second microchannel microfabricated in the interior surface of the second support body half, wherein each of the microchannels is so arranged as to provide the mirror image of the other;
• a sample processing compartment comprising an elongate bore formed by aligning the interior surfaces of the support body halves in facing abutment with each other whereby the microchannels define the elongate bore;
• an inlet port and an outlet port communicating with the sample processing compartment, the ports enabling the downstream passage of fluid from an external source through the sample processing compartment;
• and
• downstream from the sample processing compartment and in fluid communication therewith, an NMR detection compartment around which is an NMR rf microcoil, wherein the NMR detectioncompartment and the NMR rf microcoil are housed within the support body.

An integrated device for sample preparation and NMR detection, comprising:

• (a) a miniaturized total analysis system for liquid phase sample preparation and detection, comprising
• a microfabricated support body having first and second component halves each having substantially planar opposing interior and exterior surfaces,
• a first microchannel microfabricated in the interior surface of the first support body half and a second microchannel microfabricated in the interior surface of the second support body half, wherein each of the microchannels is so arranged as to provide the mirror image of the other,
• an elongate bore formed by aligning the interior surfaces of the support body halves in facing abutment with each other whereby the microchannels define the elongate bore,
• an inlet port and an outlet port communicating with the elongate bore, the ports enabling the downstream passage of fluid from an external source through the elongated bore
• downstream from the elongate bore and in fluid communication therewith, an NMR detection compartment around which is an NMR rf microcoil, wherein the NMR detection compartment and the NMR rf microcoil are housed within the support body;
• and
• (b) a magnet configured to accept the miniaturized total analysis system, wherein the device is capable of generating an NMR spectrum.

An integrated device for sample preparation and NMR detection, comprising:

• (a) a miniaturized total analysis system for liquid phase sample preparation and detection, comprising
• a microfabricated support body having first and second component halves each having substantially planar opposing interior and exterior surfaces,
• a first microchannel microfabricated in the interior surface of the first support body half and a second microchannel microfabricated in the interior surface of the second support body half, wherein each of the microchannels is so arranged as to provide the mirror image of the other,
• a sample processing compartment comprising an elongate bore formed by aligning the interior surfaces of the support body halves in facing abutment with each other whereby the microchannels define the elongate bore,
• an inlet port and an outlet port communicating with the sample processing compartment, the ports enabling the downstream passage of fluid from an external source through the sample processing compartment, and
• downstream from the sample processing compartment and in fluid communication therewith, an NMR detection compartment around which is an NMR rf microcoil, wherein the NMR detection compartment and the NMR rf microcoil arc housed within the support body;
• and
• (b) a magnet configured to accept the miniaturized total analysis system, wherein the device is capable of generating an NMR spectrum.

A miniaturized total analysis system for liquid phase sample preparation and detection comprising:

• a microfabricated support body having first and second substantially planar opposing surfaces wherein the support body has a microchannel microfabricated in the first planar surface;
• a cover plate arranged over the first planar surface, wherein the cover plate in combination with the first microchannel forms a sample processing compartment;
• an inlet port and an outlet port communicating with the sample processing compartment, wherein the inlet and outlet ports enable downstream passage of fluid from an external source through the sample processing compartment;
• and
• downstream from the sample processing compartment, a module comprising an NMR detection compartment and an NMR rf microcoil, the module being insertable into the support body such that the NMR detection compartment is in fluid communication with the sample processing compartment.

A miniaturized total analysis system for liquid phase sample preparation and detection, comprising:

• a microfabricated support body having first and second component halves each having substantially planar opposing interior and exterior surfaces;
• a first microchannel microfabricated in the interior surface of the first support body half and a second microchannel microfabricated in the interior surface of the second support body half, wherein each of the microchannels is so arranged as to provide the mirror image of the other;
• an elongate bore formed by aligning the interior surfaces of the support body halves in facing abutment with each other whereby the microchannels define the elongate bore;
• an inlet port and an outlet port communicating with the elongate bore, the ports enabling the downstream passage of fluid from an external source through the elongate bore;
• and
• downstream from the elongate bore, a module comprising an NMR detection compartment and an NMR rf microcoil, the module being insertable into the support body such that the NMR detection compartment is in fluid communication with the elongate bore.

A miniaturized total analysis system for liquid phase sample preparation and detection, comprising;

• a microfabricated support body having first and second component halves each having substantially planar opposing interior and exterior surfaces;
• a first microchannel microfabricated in the interior surface of the first support body half and a second microchannel microfabricated in the interior surface of the second support body half, wherein each of the microchannels is so arranged as to provide the mirror image of the other;
• a sample processing compartment comprising an elongate bore formed by aligning the interior surfaces of the support body halves in facing abutment with each other whereby the microchannels define the elongate bore;
• an inlet port and an outlet port communicating with the sample processing compartment, the ports enabling the downstream passage of fluid from an external source through the sample processing compartment;
• and
• downstream from the sample processing compartment, a module comprising an NMR detection compartment and an NMR rf microcoil, the module being insertable into the support body such that the NMR detection compartment is in fluid communication with the sample processing compartment.

An integrated device for sample preparation and NMR detection, comprising:

• (a) a miniaturized total analysis system for liquid phase sample preparation and detection, comprising:
• a microfabricated support body having first and second component halves each having substantially planar opposing interior and exterior surfaces;
• a first microchannel microfabricated in the interior surface of the first support body half and a second microchannel microfabricated in the interior surface of the second support body half, wherein each of the microchannels is so arranged as to provide the mirror image of the other;
• an elongate bore formed by aligning the interior surfaces of the support body halves in facing abutment with each other whereby the microchannels define the elongate bore;
• an inlet port and an outlet port communicating with the elongate bore, the ports enabling the downstream passage of fluid from an external source through the elongate bore;
• and
• downstream from the elongate bore, a module comprising an NMR detection compartment and an NMR rf microcoil, the module being insertable into the support body such that the NMR detection compartment is in fluid communication with the elongate bore;
• and
• (b) a magnet configured to accept the miniaturized total analysis system, wherein the device is capable of generating an NMR spectrum.

An integrated device for sample preparation and NMR detection, comprising:

• (a) a miniaturized total analysis system for liquid phase sample preparation and detection, comprising:
• a microfabricated support body having first and second component halves each having substantially planar opposing interior and exterior surfaces;
• a first microchannel microfabricated in the interior surface of the first support body half and a second microchannel microfabricated in the interior surface of the second support body half, wherein each of the microchannels is so arranged as to provide the mirror image of the other;
• a sample processing compartment comprising an elongate bore formed by aligning the interior surfaces of the support body halves in facing abutment with each other whereby the microchannels define the elongate bore;
• an inlet port and an outlet port communicating with the sample processing compartment, the ports enabling the downstream passage of fluid from an external source through the sample processing compartment;
• and
• downstream from the sample processing compartment, a module comprising an NMR detection compartment and an NMR rf microcoil, the module being insertable into the support body such that the NMR detection compartment is in fluid communication with the sample processing compartment;
• and
• (b) a magnet configured to accept the miniaturized total analysis system, wherein the device is capable of generating an NMR spectrum.